袁必鋒

袁必鋒 ，博士、教授，美國加州大學河濱分校化學系博士後，武漢大學化學與分子科學學院教授/博導

基本介紹

• 中文名：袁必鋒
• 國籍：中國
• 畢業院校：武漢大學
• 學位/學歷：博士
• 職業：教師
• 專業方向：生物分析化學/ 色譜-質譜分析
• 職務：博導
• 職稱：教授

基本情況

教育與研究經歷

1997.9-2001.6 武虹己潤己漢大學 生命科學學院 生物化學專業 學士

2001.9-2006.6 武漢大學 生命科學學院 生物物理專業 博士

2006.11-2007.6新加坡國立大學 生物系 博士後

2007.7-2011.1 美國加州大學河濱分校 化學系 博士後

2011.2 至今 武漢大學 化學與分子科學學院 教授/博導

主授課程

本科生課程：《化學生物學》

研究領域

研究領域

1. DNA/RNA甲基化（DNA/RNA methylation and demethylation）

DNA/RNA甲基化不僅在基因表達、調控等方面有重要作用，而且在腫瘤、免疫等許多疾病的發生和防治中也具有十分重要生物學功能。本課題組通過建立高靈敏的分析方法，斷臭宙利用各種分析化學的手段，對DNA/RNA甲基化的和去甲基化的作用機制進行探索。

2. 代謝組分析（Metabolomics）晚悼霸

發展基於質譜技術的代謝組學研究方法，並結合棄漿籃相應的生物學手段，探索代謝物在疾病發生髮展過程中的生理功能，尋找和發現新的有價值的生物標誌物。

3. 核酸損傷和修復（寒嘗灑厚Nucleic Acid Damage and Repair）

核酸損傷與基因突變、衰老、腫瘤發生等都有密切的關係。利用質譜技術以及相關的生物學手段，對細胞核心酸損傷的複製、轉錄、修復機制進行深入研究，有助於人們進一步認識各種疾病、衰老以及腫瘤的本質，為疾病的防治以及新藥的開發提供實驗和理論基礎。

科研項目

1. 自然科學基金青年基金（21205091）

2. 自然科學基金海外及港澳學者聯合基金（21228501）

3. 國家重點基礎研究婚請發展計畫（灑懂斷973 計畫）子課題（2012CB720601）

4. 教育部高等學校博士學科點專項科研基金（20120141120037）

學術論文

代表性論文

1.Yuan, B.F.* and Feng, Y.Q. (2014) Recent advances in the analysis of 5-methylcytosine and its oxidation products. TrAC-Trend Anal Chem, 54, 24-35.

2.Xing, X.W., Tang, F., Wu, J., Chu, J.M., Feng, Y.Q., Zhou, X.* and Yuan, B.F.* (2014) Sensitive detection of DNA methyltransferase activity based on exonuclease-mediated target recycling. Anal Chem, 86, 11269-11274.

3.Tang, Y., Xiong, J., Jiang, H.P., Zheng, S.J., Feng, Y.Q.* and Yuan, B.F.* (2014) Determination of oxidation products of 5-methylcytosine in plants by chemical derivatization coupled with liquid chromatography/tandem mass spectrometry analysis. Anal Chem, 86, 7764-7772.

4.Qi, B.L., Liu, P., Wang, Q.Y., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Derivatization for liquid chromatography-mass spectrometry. TrAC-Trend Anal Chem, 59, 121-132.

5.Liu, P., Huang, Y.Q., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Profiling of thiol-containing compounds by stable isotope labeling double precursor ion scan mass spectrometry. Anal Chem, 86, 9765-9773.

6.Wang, S.T., Huang, W., Lu, W., Yuan, B.F.* and Feng, Y.Q.* (2013) TiO2-Based Solid Phase Extraction Strategy for Highly Effective Elimination of Normal Ribonucleosides before Detection of 2-Deoxynucleosides/Low-Abundance 2-O-Modified Ribonucleosides. Anal Chem, 85, 10512-10518.

7.Tang, Y., Chu, J.M., Huang, W., Xiong, J., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Hydrophilic material for the selective enrichment of 5-hydroxymethylcytosine and its liquid chromatography-tandem mass spectrometry detection. Anal Chem, 85, 6129-6135.

8.Chen, M.L., Shen, F., Huang, W., Qi, J.H., Wang, Y., Feng, Y.Q.*, Liu, S.M.* and Yuan, B.F.* (2013) Quantification of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Carcinoma Tissues by Capillary Hydrophilic-Interaction Liquid Chromatography/Quadrupole TOF Mass Spectrometry. Clin Chem, 59, 824-832.

9.Chen, M.L., Liu, Y.L., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Preparation of a Hyper-cross-linked Polymer Monolithic Column and Its Application to the Sensitive Determination of Genomic DNA Methylation. Chemistry, 19, 1035-1041.

10.Tang, Y., Gao, X.D., Wang, Y., Yuan, B.F.* and Feng, Y.Q.* (2012) Widespread existence of Cytosine methylation in yeast DNA measured by gas chromatography/mass spectrometry. Anal Chem, 84, 7249-7255.

11.Yuan, B.F., Zhang, J., Wang, H., Xiong, L., Cai, Q., Wang, T., Jacobsen, S., Pradhan, S. and Wang, Y.* (2011) 6-Thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1. Cancer Res, 71, 1904-1911.

12.Yuan, B.F., Wang, J., Cao, H., Sun, R. and Wang, Y.* (2011) High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencing. Nucleic Acids Res, 39, 5945-5954.

13.Yuan, B.F., O Connor, T.R. and Wang, Y.* (2010) 6-Thioguanine and S(6)-Methylthioguanine Are Mutagenic in Human Cells. ACS Chem. Biol., 5, 1021-1027.

14.Yuan, B.F., Cao, H., Jiang, Y., Hong, H. and Wang, Y.* (2008) Efficient and accurate bypass of N-(1-carboxyethyl)-2-deoxyguanosine by DinB DNA polymerase in vitro and in vivo. Proc. Natl. Acad. Sci. U S A., 105, 8679-8684.

15.Yuan, B.F., Zhuang, X.Y., Hao, Y.H. and Tan, Z.* (2008) Kinetics of base stacking-aided DNA hybridization. Chem Commun (Camb), 6600-6602.

16.Yuan, B.F., Hao, Y.H. and Tan, Z.* (2004) Universal sensing strategy for the detection of nucleic acid targets by optical biosensor based on surface plasmon resonance. Clin. Chem., 50, 1057-1060.

獲獎榮譽

2011 “楚天學者”特聘教授

3.Tang, Y., Xiong, J., Jiang, H.P., Zheng, S.J., Feng, Y.Q.* and Yuan, B.F.* (2014) Determination of oxidation products of 5-methylcytosine in plants by chemical derivatization coupled with liquid chromatography/tandem mass spectrometry analysis. Anal Chem, 86, 7764-7772.

4.Qi, B.L., Liu, P., Wang, Q.Y., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Derivatization for liquid chromatography-mass spectrometry. TrAC-Trend Anal Chem, 59, 121-132.

5.Liu, P., Huang, Y.Q., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Profiling of thiol-containing compounds by stable isotope labeling double precursor ion scan mass spectrometry. Anal Chem, 86, 9765-9773.

6.Wang, S.T., Huang, W., Lu, W., Yuan, B.F.* and Feng, Y.Q.* (2013) TiO2-Based Solid Phase Extraction Strategy for Highly Effective Elimination of Normal Ribonucleosides before Detection of 2-Deoxynucleosides/Low-Abundance 2-O-Modified Ribonucleosides. Anal Chem, 85, 10512-10518.

7.Tang, Y., Chu, J.M., Huang, W., Xiong, J., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Hydrophilic material for the selective enrichment of 5-hydroxymethylcytosine and its liquid chromatography-tandem mass spectrometry detection. Anal Chem, 85, 6129-6135.

8.Chen, M.L., Shen, F., Huang, W., Qi, J.H., Wang, Y., Feng, Y.Q.*, Liu, S.M.* and Yuan, B.F.* (2013) Quantification of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Carcinoma Tissues by Capillary Hydrophilic-Interaction Liquid Chromatography/Quadrupole TOF Mass Spectrometry. Clin Chem, 59, 824-832.

9.Chen, M.L., Liu, Y.L., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Preparation of a Hyper-cross-linked Polymer Monolithic Column and Its Application to the Sensitive Determination of Genomic DNA Methylation. Chemistry, 19, 1035-1041.

10.Tang, Y., Gao, X.D., Wang, Y., Yuan, B.F.* and Feng, Y.Q.* (2012) Widespread existence of Cytosine methylation in yeast DNA measured by gas chromatography/mass spectrometry. Anal Chem, 84, 7249-7255.

11.Yuan, B.F., Zhang, J., Wang, H., Xiong, L., Cai, Q., Wang, T., Jacobsen, S., Pradhan, S. and Wang, Y.* (2011) 6-Thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1. Cancer Res, 71, 1904-1911.

12.Yuan, B.F., Wang, J., Cao, H., Sun, R. and Wang, Y.* (2011) High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencing. Nucleic Acids Res, 39, 5945-5954.

13.Yuan, B.F., O Connor, T.R. and Wang, Y.* (2010) 6-Thioguanine and S(6)-Methylthioguanine Are Mutagenic in Human Cells. ACS Chem. Biol., 5, 1021-1027.

14.Yuan, B.F., Cao, H., Jiang, Y., Hong, H. and Wang, Y.* (2008) Efficient and accurate bypass of N-(1-carboxyethyl)-2-deoxyguanosine by DinB DNA polymerase in vitro and in vivo. Proc. Natl. Acad. Sci. U S A., 105, 8679-8684.

15.Yuan, B.F., Zhuang, X.Y., Hao, Y.H. and Tan, Z.* (2008) Kinetics of base stacking-aided DNA hybridization. Chem Commun (Camb), 6600-6602.

16.Yuan, B.F., Hao, Y.H. and Tan, Z.* (2004) Universal sensing strategy for the detection of nucleic acid targets by optical biosensor based on surface plasmon resonance. Clin. Chem., 50, 1057-1060.

獲獎榮譽

2011 “楚天學者”特聘教授