杜文斌，男，畢業於浙江大學，博士學位，現為研究員。
基本介紹
- 中文名：杜文斌
- 職業：研究員
- 畢業院校：浙江大學
- 主要成就：
- 性別：男
個人履歷,教育經歷,工作經歷,獲獎情況,研究領域,研究內容,學術成果,
個人履歷
教育經歷
1998-2002浙江大學化學系，學士
2002-2007浙江大學化學系微分析系統研究所，博士。
工作經歷
2007-2011美國芝加哥大學化學系及動態生物物理研究所Rustem F. Ismagilov實驗室，博士後；
2011-2012 中國人民大學化學系，特聘研究員，博士生導師；
2013-至今 中國科學院微生物研究所，研究員，博士生導師；
獲獎情況
2012年：入選第七批"千人計畫(青年項目)"
2012年：中國科學院“百人計畫”B類。
研究領域
微流控生化分析晶片及微生物學套用
研究內容
幾何尺度達到以微米和納米的微納加工技術，是套用廣泛、發展迅速的一門多領域交叉的新學科，對於現代科學技術具有革命性的意義。以微納加工技術為依託，微流控(Microfluidics) 通過微尺度通道及結構，可以精確的控制和測量微量流體中的生化分子、細胞、微生物個體等及其所處的微環境，拓展了人類對微觀生命世界的認識和操控能力，也為微生物相關技術的發展提供了新的機遇。本課題組從微流控器件的設計、製備和集成出發，致力於建立微生物學“晶片實驗室”(Lab On a Chip)，加快對微生物世界的多樣性和功能的探索和認識。近期主要研究課題包括：
(1)基於陣列液滴微流控技術，實現高通量環境和極端環境微生物單細胞分離、培養和鑑定，以及定向篩選高性能、高特異性微生物菌種。
(2)基於連續層流和靜態擴散的微流控的定量微生物趨化研究。
(3)結合微流控捕獲技術和陣列PCR技術，實現環境和臨床微生物的捕獲和高靈敏度檢測。
(4)開發毛細管等電聚焦和二維電泳分析系統，套用於超微量的蛋白組分析。
學術成果
發表論文
(1) Shen, F.; Sun, B.; Kreutz, J. E.; Davydova, E. K.; Du, W. B.; Reddy, P. L.; Joseph, L. J.; Ismagilov, R. F.*, Multiplexed Quantification of Nucleic Acids with Large Dynamic Range Using Multivolume Digital RT-PCR on a Rotational SlipChip Tested with HIV and Hepatitis C Viral Load, Journal of the American Chemical Society 2011, 133, 17705.
(2) Pompano, R. R.; Liu, W. S.; Du, W. B.; Ismagilov, R. F.*, Microfluidics Using Spatially Defined Arrays of Droplets in One, Two, and Three Dimensions, Annual Review of Analytical Chemistry, Vol 4; Cooks, R. G. Y. E. S., Ed. 2011; Vol. 4, p 59.
(3) Kim, H. J.; Du, W. B.; Ismagilov, R. F.*, Complex function by design using spatially pre-structured synthetic microbial communities: degradation of pentachlorophenol in the presence of Hg(II), Integrative Biology 2011, 3, 126.
(4) Gu, S. Q.; Zhang, Y. X.; Zhu, Y.; Du, W. B.; Yao, B.; Fang, Q.*, Multifunctional Picoliter Droplet Manipulation Platform and Its Application in Single Cell Analysis, Analytical Chemistry 2011, 83, 7570.
(5) Du, W. B.; Sun, M.; Gu, S. Q.; Zhu, Y.; Fang, Q.*, Automated Microfluidic Screening Assay Platform Based on Drop Lab. Analytical Chemistry 2010, 82, 9941.
(6) Li, L.; Du, W. B.*; Ismagilov, R. F.*, Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods, Journal of the American Chemical Society 2010, 132, 112.
(7) Li, L.; Du, W. B.; Ismagilov, R. F.*, User-Loaded SlipChip for Equipment-Free Multiplexed Nanoliter-Scale Experiments, Journal of the American Chemical Society 2010, 132, 106.
(8) Kreutz, J. E.; Shukhaev, A.; Du, W. B.; Druskin, S.; Daugulis, O.; Ismagilov, R. F.*, Evolution of Catalysts Directed by Genetic Algorithms in a Plug-Based Microfluidic Device Tested with Oxidation of Methane by Oxygen, Journal of the American Chemical Society 2010, 132, 3128.
(9) Shen, F.; Du, W. B.; Davydova, E. K.; Karymov, M. A.; Pandey, J.; Ismagilov, R. F.*, Nanoliter Multiplex PCR Arrays on a SlipChip, Analytical Chemistry 2010, 82, 4606.
(10) Shen, F.; Du, W. B.; Kreutz, J. E.; Fok, A.; Ismagilov, R. F.*, Digital PCR on a SlipChip, Lab on a Chip, 2010, 10, 2666.
(11) Liu, W. S.; Chen, D. L.; Du, W. B.; Nichols, K. P.; Ismagilov, R. F.*, Slip Chip for Immunoassays in Nanoliter Volumes, Analytical Chemistry 2010, 82, 3276.
(12) Du, W. B.; Li, L.; Nichols, K. P.; Ismagilov, R. F.*, SlipChip, Lab on a Chip 2009, 9, 2286.
(13) Zhang, T.; Fang, Q.*; Du, W. B.; Fu, J. L., Microfluidic Picoliter-Scale Translational Spontaneous Sample Introduction for High-Speed Capillary Electrophoresis, Analytical Chemistry 2009, 81, 3693.
(14) Liu, W.; Kim, H. J.; Lucchetta, E. M.; Du, W. B.; Ismagilov, R. F.*, Isolation, incubation, and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement, Lab on a Chip 2009, 9, 2153.
(15)Chen, D. L.†; Du, W. B.† (equal contribution); Liu, Y.†; Liu, W.†; Kuznetsov, A.; Mendez, F. E.; Philipson, L. H.; Ismagilov, R. F.*, The chemistrode: A droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution, Proceedings of the National Academy of Sciences of the United States of America 2008, 105, 16843.
(16)Du, W. B.; Fang, Q.*; Fang, Z. L., Microfluidic sequential injection analysis in a short capillary, Analytical Chemistry 2006, 78, 6404.
(17)Sun, M.; Du, W. B.; Fang, Q.*, Microfluidic liquid-liquid extraction system based on stopped-flow technique and liquid core waveguide capillary, Talanta 2006, 70, 392.
(18)Du, W. B.; Fang, Q.*; He, Q. H.; Fang, Z. L., High-throughput nanoliter sample introduction microfluidic chip-based flow injection analysis system with gravity-driven flows, Analytical Chemistry 2005, 77, 1330.
(19)He, Q. H.; Fang, Q.; Du, W. B.; Huang, Y. Z.; Fang, Z. L., An automated electrokinetic continuous sample introduction system for microfluidic chip-based capillary electrophoresis, Analyst 2005, 130, 1052.
(20)Du, W. B.; Fang, Q.*; Fang, Z. L., A microfluidic chip for absorbance measurements with long optical path-length based on a liquid-core waveguide technique,Chemical Journal of Chinese Universities-Chinese 2004, 25, 610.
(21)Fang, Q.*; Du, W. B.; He, Q. H.; Fang, Z. L., High throughput sample introduction system for microfluidic chips,Chemical Journal of Chinese Universities-Chinese 2004, 25, 1442.
(2) Pompano, R. R.; Liu, W. S.; Du, W. B.; Ismagilov, R. F.*, Microfluidics Using Spatially Defined Arrays of Droplets in One, Two, and Three Dimensions, Annual Review of Analytical Chemistry, Vol 4; Cooks, R. G. Y. E. S., Ed. 2011; Vol. 4, p 59.
(3) Kim, H. J.; Du, W. B.; Ismagilov, R. F.*, Complex function by design using spatially pre-structured synthetic microbial communities: degradation of pentachlorophenol in the presence of Hg(II), Integrative Biology 2011, 3, 126.
(4) Gu, S. Q.; Zhang, Y. X.; Zhu, Y.; Du, W. B.; Yao, B.; Fang, Q.*, Multifunctional Picoliter Droplet Manipulation Platform and Its Application in Single Cell Analysis, Analytical Chemistry 2011, 83, 7570.
(5) Du, W. B.; Sun, M.; Gu, S. Q.; Zhu, Y.; Fang, Q.*, Automated Microfluidic Screening Assay Platform Based on Drop Lab. Analytical Chemistry 2010, 82, 9941.
(6) Li, L.; Du, W. B.*; Ismagilov, R. F.*, Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods, Journal of the American Chemical Society 2010, 132, 112.
(7) Li, L.; Du, W. B.; Ismagilov, R. F.*, User-Loaded SlipChip for Equipment-Free Multiplexed Nanoliter-Scale Experiments, Journal of the American Chemical Society 2010, 132, 106.
(8) Kreutz, J. E.; Shukhaev, A.; Du, W. B.; Druskin, S.; Daugulis, O.; Ismagilov, R. F.*, Evolution of Catalysts Directed by Genetic Algorithms in a Plug-Based Microfluidic Device Tested with Oxidation of Methane by Oxygen, Journal of the American Chemical Society 2010, 132, 3128.
(9) Shen, F.; Du, W. B.; Davydova, E. K.; Karymov, M. A.; Pandey, J.; Ismagilov, R. F.*, Nanoliter Multiplex PCR Arrays on a SlipChip, Analytical Chemistry 2010, 82, 4606.
(10) Shen, F.; Du, W. B.; Kreutz, J. E.; Fok, A.; Ismagilov, R. F.*, Digital PCR on a SlipChip, Lab on a Chip, 2010, 10, 2666.
(11) Liu, W. S.; Chen, D. L.; Du, W. B.; Nichols, K. P.; Ismagilov, R. F.*, Slip Chip for Immunoassays in Nanoliter Volumes, Analytical Chemistry 2010, 82, 3276.
(12) Du, W. B.; Li, L.; Nichols, K. P.; Ismagilov, R. F.*, SlipChip, Lab on a Chip 2009, 9, 2286.
(13) Zhang, T.; Fang, Q.*; Du, W. B.; Fu, J. L., Microfluidic Picoliter-Scale Translational Spontaneous Sample Introduction for High-Speed Capillary Electrophoresis, Analytical Chemistry 2009, 81, 3693.
(14) Liu, W.; Kim, H. J.; Lucchetta, E. M.; Du, W. B.; Ismagilov, R. F.*, Isolation, incubation, and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement, Lab on a Chip 2009, 9, 2153.
(15)Chen, D. L.†; Du, W. B.† (equal contribution); Liu, Y.†; Liu, W.†; Kuznetsov, A.; Mendez, F. E.; Philipson, L. H.; Ismagilov, R. F.*, The chemistrode: A droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution, Proceedings of the National Academy of Sciences of the United States of America 2008, 105, 16843.
(16)Du, W. B.; Fang, Q.*; Fang, Z. L., Microfluidic sequential injection analysis in a short capillary, Analytical Chemistry 2006, 78, 6404.
(17)Sun, M.; Du, W. B.; Fang, Q.*, Microfluidic liquid-liquid extraction system based on stopped-flow technique and liquid core waveguide capillary, Talanta 2006, 70, 392.
(18)Du, W. B.; Fang, Q.*; He, Q. H.; Fang, Z. L., High-throughput nanoliter sample introduction microfluidic chip-based flow injection analysis system with gravity-driven flows, Analytical Chemistry 2005, 77, 1330.
(19)He, Q. H.; Fang, Q.; Du, W. B.; Huang, Y. Z.; Fang, Z. L., An automated electrokinetic continuous sample introduction system for microfluidic chip-based capillary electrophoresis, Analyst 2005, 130, 1052.
(20)Du, W. B.; Fang, Q.*; Fang, Z. L., A microfluidic chip for absorbance measurements with long optical path-length based on a liquid-core waveguide technique,Chemical Journal of Chinese Universities-Chinese 2004, 25, 610.
(21)Fang, Q.*; Du, W. B.; He, Q. H.; Fang, Z. L., High throughput sample introduction system for microfluidic chips,Chemical Journal of Chinese Universities-Chinese 2004, 25, 1442.
承擔科研項目情況
本研究組的工作獲得國家自然科學基金項目、國家863計畫項目、中國大洋協會項目等的支持。
目前在研的主要科研項目
1.中組部“青年千人”計畫項目, 2012-2015
2.中國科學院“百人計畫”，2013-2015
3.國家自然科學基金青年項目 ” 用於細菌趨化性定量檢測和篩選的液滴微流控平台及其套用“（主持），2013-2015
4.中國科學院微生物研究所微生物資源前期開發國家重點實驗室課題，“微流控液滴技術在極端環境微生物的單細胞培養與最佳化篩選中的套用”（主持），2012-2015
5.國家自然科學基金重點項目 “叢毛單胞菌等對芳環類污染物的趨化、降解及其在生物修復中的作用“（參加），2013-2017
6.國際海域資源調查與開發“十二五”，“深海典型環境微生物的群落特徵與套用潛力發掘”（子課題），2011-2015
3.國家自然科學基金青年項目 ” 用於細菌趨化性定量檢測和篩選的液滴微流控平台及其套用“（主持），2013-2015
4.中國科學院微生物研究所微生物資源前期開發國家重點實驗室課題，“微流控液滴技術在極端環境微生物的單細胞培養與最佳化篩選中的套用”（主持），2012-2015
5.國家自然科學基金重點項目 “叢毛單胞菌等對芳環類污染物的趨化、降解及其在生物修復中的作用“（參加），2013-2017
6.國際海域資源調查與開發“十二五”，“深海典型環境微生物的群落特徵與套用潛力發掘”（子課題），2011-2015
