《Strategies for Protein Purification and Characterization》是1995年出版的一本圖書，作者是 Marshak, Daniel R.、 Daniel Marshak; Burgess, Richard R.。
基本介紹
- 中文名：Strategies for Protein Purification and Characterization
- 作者：Marshak, Daniel R.、 Daniel Marshak; Burgess, Richard R.
- 語言：英語
- 出版時間：1995年1月
- ISBN：9780879693855
內容簡介
Investigators who have identified and cloned a gene of interest often want to isolate and characterize the protein product, yet the methods required are notoriously difficult for the inexperienced. This manual has been adapted from a course held at the Cold Spring Harbour Laboratory in New York, to teach scientists how to execute the major protein techniques by applying them to four distinct, representative types of molecule: a regulatory protein, a DNA-binding protein, a recombinant protein, and a membrane-bound receptor. This manual covers a variety of bulk fractionation, electrophoretic, and chromatographic techniques. Step-by-step protocols are accompanied by troubleshooting advice and guidance on generalizing the techniques for other classes and types of protein. The emphasis throughout is on strategies for purification and characterization rather than automated instrumental analysis. The book should be of use to specialists in genetics, microbiology, neuroscience, and cell biology, who wish to develop expertise in working with proteins.
