Peptide mapping簡稱肽譜，主要通過利用蛋白水解酶（protease）將未知肽鏈水解成短片段，這些片段可以通過PAGE進行分離，由此可得到一系列的指紋圖譜（fingerprint）。
基本介紹
- 中文名：肽譜
- 外文名：Peptide mapping
肽譜可以顯示出未知蛋白片段上的裂解位點，或者通過我們已有的樣本信息基於指紋圖譜可以鑑定出未知的蛋白。
（謝絕轉載，CAS）
Peptide mapping簡稱肽譜，主要通過利用蛋白水解酶（protease）將未知肽鏈水解成短片段，這些片段可以通過PAGE進行分離，由此可得到一系列的指紋圖譜（fingerprint）。
Peptide mapping Peptide mapping簡稱肽譜，主要通過利用蛋白水解酶（protease）將未知肽鏈水解成短片段，這些片段可以通過PAGE進行分離，由此可得到一系列的指紋圖譜（fingerprint）。肽譜可以顯示出未知蛋白片段上的裂解位點，或者通過我們已有的樣本信息基於指紋圖譜可以鑑定出未知的蛋白。（謝絕轉載，CAS）
《糖結合型海參膠原肽生化特性及其生物活性的研究》是依託中國海洋大學，由薛長湖擔任項目負責人的面上項目。中文摘要 本項目研究內容是：以海參為研究對象，採用生化分離技術，通過SDS-PAGE、IEF、peptide mapping等技術鑑定糖結合型膠原蛋白類型。採用海參中多種自溶蛋白酶和商業酶對膠原蛋白進行降解製備不同的活性多肽，...
CH, Yang YY, Li QM, Guo JQ, Qiao HX, Xi J, Zhao D, Xing GX, Wang ZL, Wang SH, Xiao ZJ, Li XW, Deng RG. 2005. Identification of neutralizing epitopes on the VP2 protein of infectious bursal disease virus by phage displayed heptapeptide library screening and synthetic peptide mapping...
by N—terminal microsequencing and mass spectrometric peptide mapping Chemical carboxyl．terminal sequence analysis of peptides and proteins using tribenzylsilyl isothiocyanate Assignment of the disulfide bonds of huwentoxin—II by Edman degradation sequencing and stepwise thiol modification C-terminal sequence...
10. Yao, Guoping; Deng, Chunhui*; Zhang, Xiangmin; Yang, Pengyuan, Efficient Tryptic Proteolysis Accelerated by Laser Radiation for Peptide Mapping in Proteome Analysis, Angewandte Chemie International Edition, 2010, 49(44), 8185-8189.Chunhui Deng, Microfluidic Devices in Nanotechnology: ...
Aiping Lu, Leonie F. Waanders, Reinaldo Almeida, Guoqing Li, Mark Allen , Jürgen Cox, Jesper V. Olsen, Tiziana Bonaldi and Matthias Mann. “Nanoelectrospray peptide mapping revisited: Composite survey spectra allow high dynamic range protein characterization without LCMS on an orbitrap mass ...
Feng CH, Yang YY, Li QM, Guo JQ, Qiao HX, Xi J, Zhao D, Xing GX, Wang ZL, Wang SH, Xiao ZJ, Li XW, Deng RG. Identification of Neutralizing Epitopes on the VP2 Protein of Infectious Bursal Disease Virus by Phage-Displayed Heptapeptide Library Screening and Synthetic Peptide Mapping....
71、Chen, Caiwei, Li, Jing, Bi, Yuhai, Yang, Limin, Meng, Shanshan, Zhou, Yuancheng, Jia, Xiaojuan, Meng, Songdong, Sun, Lei, Liu, Wenjun. Synthetic B- and T-cell epitope peptides of porcine reproductive and respiratory syndrome virus with Gp96 as adjuvant induced humoral and cell-...
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peptide peptidebond鍵 phagemids噬菌粒 phosphorylation磷酸化作用 physicalmap物理圖譜 plasmid質粒 pointmutation點突變 poly(A)tailpoly(A)尾 polymerase聚合 polyploid多倍體 positionalcloning位置性無性繁殖系化 primarytranscript初級轉錄物 primer引物 probe探針 prokaryotic原核的 promoter啟動子 protease蛋白...
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（12） A Note on the False Discovery Rate of Novel Peptides in Proteogenomics, Bioinformatics, 2015, 通訊作者 （13） Mapping native disulfide bonds at a proteome scale, Nature Methods, 2015, 通訊作者 （14） pNovo+: De Novo Peptide Sequencing Using Complementary HCD and ETD Tandem Mass ...
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[3]WH Chen, WW Guo, YX Huang*, ZQ Ma*. PepMapper: A Collaborative Web Tool for Mapping Epitopes from Affinity-Selected Peptides. PLoS ONE 2012, 7(5): e37869.[4]XW Zhao, JK Li, YX Huang*, ZQ Ma*, MH Yin*. Prediction of Bioluminescent Proteins Using Auto Covariance Transformation ...
25. Sanduo Zheng and Keqiong Ye* (2014) Purification, crystallization and preliminary X-ray diffraction analysis of Imp3 in complex with an Mpp10 peptide involved in yeast ribosome biogenesis. Acta Cryst. F90, 918-921 26. Dejian Zhou, Keqiong Ye* (2014) CLIP techniques in studying protein...
（10）中國白蛉亞科昆蟲分布情況調查，國家自然科學基金1987-1993，項目組成員2005-2006年在美國耶魯大學醫學院進修期間，參與了關於甘比亞按蚊對瘧原蟲感染的免疫調節的NIH基金項目（R01A43035, AI43053）, 進行了“QTL mapping for Tep1 in melanotic encapsulation”、“PGRP and antimicrobial peptides in Anopheles ...
6. The 10thAnniversary of Protein & Peptide Conference PepCon-2017 Fukuoka（福岡）,日本 ，2017 7. InternationalConference on Oncology & Cancer Science Sydney（悉尼）, 澳大利亞， 2017 代表論文 Suseng Ci, Wen Xia, Menghan Li, Feiyan Pan *, GUO Z * .Src-mediated phosphorylation of...
[1] Yong Lu, Didier Mekoo, OuyanKedong, XiangbingHu, Yanhua Liu, Ming Lin, Liang Jin, Rongyue Cao, Taiming Li, Zhang Yankai, Hao Fan and Liu Jingjing*.Strong humoral response elicited by a DNA vaccine targeting gastrin-releasing peptide with optimized adjuvants inhibits murine prostate ...
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2.Youyuan Li、Pei Hao、Siliang Zhang*、Yixue Li：Feature-matching Pattern-based Support Vector Machines for Robust Peptide Mass Fingerprinting，《Molecular & Cellular Proteomics》,10: 10.1074/mcp.M110.005785, 1–17, 2011(2011IF 7.398)3.李友元、莊英萍、張嗣良：生物信息軟體的自動化策略與實現，《...
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11. Tian CX, Zhang JP, Feng PZ, Lin XH, Ru XY, Zhu CH, Li GL*. Comparative analysis of transcriptome responses to injected somatostatin 3 peptide in spotted scat (Scatophagus argus). Aquaculture Reports, 2022, 23. 12. Lin XH, Huang Y, Jiang DN, Chen HP, Deng SP, Zhang YL, Du ...