李惠琳

李惠琳

李惠琳，女，博士，中山大學教授、博士生導師。2017年12月通過“中山大學百人計畫”進入中山大學藥學院工作。其研究工作以高分辨質譜技術為主要手段，開發先進的Top-Down MS技術，在整體蛋白、蛋白質機器水平上實現對蛋白的測序、蛋白翻譯後修飾位點及藥物結合位點的精準定位，進而對蛋白質機器結構和功能及蛋白-藥物作用機制進行深入研究。其研究成果發表在Nature Chemistry, Analytical Chemistry, J. Am. Soc. Mass Spectrom.等國際主流化學、分析、及質譜類雜誌。

基本介紹

  • 中文名：李惠琳
  • 畢業院校：英國華威大學
  • 學位/學歷：博士
  • 職業：教師
  • 職務：中山大學博士生導師
  • 職稱：教授
人物經歷,教育經歷,工作經歷,研究方向,科研項目,發表論文,獲獎記錄,

人物經歷

教育經歷

2009年10月至2012年12月：博士，英國華威大學化學系，導師：Peter B. O’Connor 教授；
2005年9月至2008年12月：碩士，中國科學院長春套用化學研究所，導師：劉志強 研究員；
1998年9月至2002年7月：學士，山東大學藥學院

工作經歷

2017年12月-至今 教授，博士生導師，中山大學，藥學院
2016年9月至2017年9月：博士後，法國遺傳與分子細胞生物學研究所結構生物學系，合作導師：Bruno Kieffer & Marc-Andre Delsuc教授；
2013年1月至2016年1月：博士後，美國加州大學洛杉磯分校David Geffen醫學院生物化學系，合作導師：Joseph Loo教授；
2002年7月至2009年9月：研習員，助理研究員，中國科學院長春套用化學研究所

研究方向

（1）開發及利用整合結構質譜技術（包括native top-down MS, HDX-MS, cross-linking MS等）研究蛋白質機器（蛋白-藥物、蛋白-蛋白、蛋白-DNA等）的結構和功能；
（2）開發靶向middle-down/top-down蛋白質組學技術探索蛋白翻譯後修飾（PTMs）在生命過程中的調控機制。

科研項目

中山大學“百人計畫二期”啟動項目，負責
國家自然科學基金面上項目，2019.1-2022.12，負責

發表論文

  1. H Li*. Native top-down proteomics comes into light.Sci.China LifeSci.2018, 61(11),1425–1427.
  2. H Li*,HH Nguyen, RRO Loo, I Campuzano, JA Loo*. Anintegratednative mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes.Nat. Chem.2018, 10(2):139-148
  3. H Li*,Y Sheng, W McGee, M Cammarata, D Holden, JA Loo*. Structural Characterization of Native Proteins and Protein Complexes by Electron Ionization Dissociation.Anal. Chem.2017. 89, 2731-2738
  4. I Campuzano,H Li, G Svitel, D Bagal, L Jia, R Kurzeja, H Xu, JA Loo*. Bacteriorhodopsin and Nanodisc Analysis by Orthogonal Acceleration Time-of-flight, Ion Cyclotron Resonance and OrbiTrap Analyzers.Anal. Chem.2016. 88, 12427-12436.
  5. P Wongkongkathep,H Li, X Zhang, RRO Loo, R Julian and JA Loo*. Enhancing Disulfide Bond Cleavage by UV Excitation and Electron Capture Dissociation for Top-Down Mass Spectrometry.Int. J. Mass Spectrom. 2015.390, 137-145.
  6. X Zhang,H Li, JA Loo, RR Julian*, B Moore, P Wongkongkathep, RRO Loo. Radical directed dissociation of peptides and proteins by IRMPD and SORI-CID with FTICR mass spectrometry.Rapid Commun. Mass Spectrom.2014. 28, 2729-2734.
  7. H Li, P Wongkongkathep, SL Van Orden, RRO Loo, JA Loo*. Revealing Ligand Binding Sites and Quantifying Subunit Variants of Non-Covalent Bound Protein Complexes in a Single Native Top-Down FTICR MS Experiment.J. Am. Soc. Mass Spectrom. 2014. 25, 2060-2068.
  8. H Li, JJ Wolff, SL Van Orden, JA Loo*. Native Top-Down ESI-MS of 158 kDa Protein Complex by High Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.Anal. Chem.2014, 86, 317-320 (Paper On the Cover)
  9. H Li, JR Snelling, MP Barrow, JH Scrivens, PJ Sadler, PB O’Connor*. Mass Spectrometric Strategies to Improve the Identification of Pt(II)-modification Sites on Peptides and proteins.J. Am. Soc. Mass Spectrom. 2014. 25, 1217-1227.
  10. Y Zhao, NJ Farrer,H Li, JS Butler, RJ McQuitty, A Habtemariam, F Wang, PJ Sadler*. De novo generation of singlet oxygen and ammine ligands by photoactivation of a platinum anticancer complex.Angew. Chem. 2013. 52, 1-6.
  11. Y Qi,H Li, RH Wills, P Perez-Hurtado, X Yu, DPA Kilgour, MP Barrow,C Lin,PB O’Connor*. Absorption-mode Fourier transform mass spectrometry: The effects of apodization and phasing on modified protein spectra.J. Am. Soc. Mass Spectrom. 2013, 24, 828-834.
  12. J Wei,H Li, MP Barrow,PB O’Connor*. Structural Characterization of Chlorophyll a by High Resolution Tandem Mass Spectrometry.J. Am. Soc. Mass Spectrom. 2013. 24, 753-760.
  13. Y Qi, Z Liu,H Li, PJ Sadler, PB O'Connor*. Mapping the Protein-Binding Sites for Novel Iridium(III) Anticancer Complexes Using Electron Capture Dissociation.Rapid Commun. Mass Spectrom.2013,27, 2028-2032.
  14. Y Zhao, JA Woods, NJ Farrer, KS Robinson, J Pracharova, J Kasparkova, O Novakova,H Li, L Salassa, AM Pizarro, GJ Clarkson, L Song, V Brabec, PJ Sadler*. Diazido mixed-amine platinum(IV) anticancer complexes activatable by visible-light form novel DNA adducts.Chem. Eur. J.2013, 19, 9578-9591.
  15. H Li, PB O’Connor*.Electron Capture Dissociation of Disulfide, Sulfur-Selenium, and Diselenide Bound Peptides.J. Am. Soc. Mass Spectrom.2012, 23, 2001-2010.
  16. H Li#, SA Wells#, JE Jimenez-Roldan, RA Römer, Y Zhao, PJ Sadler, PB O’Connor*. Protein Flexibility is Key to Cisplatin Cross-linking in Calmodulin.Prot. Sci.2012. 21, 1269-1279.
  17. Y Qi, MP Barrow,H Li, JE Meier, SL Van Orden, CJ Thompson, PB O'Connor*. Absorption-mode: The Next Generation of Fourier Transform Mass Spectra.Anal. Chem.2012. 84, 2923–2929.
  18. H Zhou,H Li, Z Zheng, F Song, J Xing, Z Liu*, S Liu*. Screening for α-Glucosidase Inhibitors from Coptidis-rehmanniae Herb Couple by Using Ultrafiltration Liquid Chromatography/Mass Spectrometry.J. Liq. Chrom. Rel. Technol.2012, 35, 1-14.
  19. H Li, TY Lin, SL Van Orden, Y Zhao, MP Barrow, AM Pizarro, Y Qi, PJ Sadler, PB O'Connor*. Use of Top-down and Bottom-up Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Mapping Calmodulin Sites Modified by Platinum Anticancer Drugs.Anal. Chem.2011, 83, 9507-9515.
  20. H Li, Y Zhao, HIA Phillips, Y Qi, TY Lin, PJ Sadler, PB O’Connor*. Mass Spectrometry Evidence for Cisplatin as a Protein Cross-linking Reagent.Anal. Chem.2011, 83, 5369-5376.
  21. Y Qi, MP Barrow, SL Van Orden, CJ Thompson,H Li, P Perez-Hurtado, PB O'Connor*. Variation of the Fourier Transform Mass Spectra Phase Function with Experimental Parameters.Anal. Chem.2011, 83, 8477-8483.
  22. W Liu, S Liu,H Li, F Song, Z Liu, S Liu*. Binding of Alpha 1-acid Glycoprotein with Aconitum Alkaloids: An Investigation by IF-MALDI-FT-MS.Rapid Commun. Mass Spectrom.2011, 25, 973-978.
  23. S Liu, J Yan,H Li, F Song, Z Liu, Z Liu, S Liu. Studies on Chemical Constituents of Compound Indigowoad Root Granule by Mass Spectrometry.Chem. J. Chinese U.2010, 31, 1137-1142.
  24. H Li, F Song, J Xing, Z Liu*, S Liu*. Screening and Structures Characterization of α-Glucosidase Inhibitors from Hawthorn Leaves Flavonoids Extract by Ultrafiltration LC-DAD-MSnand SORI-CID FT ICR MS.J. Am. Soc. Mass Spectrom.2009, 20, 1496-1503.
  25. X Meng,H Li, F Song, C Liu, Z Liu, S Liu. Studies on Triterpenoids and Flavones inGlycyrrhiza uralensis Fisch. by HPLC-ESI-MSnand FT-ICR-MSn.Chinese J. Chem. 2009, 27, 299-305.
  26. H Li, F Song, Z Zheng, Z Liu*, S Liu*. Characterization of Oligsaccharides and Phenolid Acids in the Chinese Herb Tanshen by ESI-FT ICR MS.J. Mass Spectrom.2008, 43, 1545-1552.
  27. H Yue, Z Pi,H Li, F Song, Z Liu, S Liu. Studies on the Stability of Diester-diterpenoid Alkaloids from theGenus Aconitum L.by High Performance Liquid Chromatography Combined with Electrospray Ionisation Tandem Mass Spectrometry (HPLC/ESI/MSn).Phytochem. Anal.2008, 19, 141-147.
  28. Y Liu, Z Liu,H Li, F Song, S Liu. Studies on Diterpenoids Constituents from Euphorbia Kansui by Electrospray Ionization Multi-Stage Tandem Mass Spectrometry.Chem. J. Chinese U.2008, 29, 1727-1735.
  29. X Qiu, M Cui,H Li, Z Liu, S Liu. Prompt Disulfide Fragmentations of Disulfide-containing Proteins in a Matrix-Assisted Laser Desorption/ Ionization Source.Rapid Commun. Mass Spectrom. 2007, 21, 3520-3525.
  30. Y Zhao, F Song, H Yue, X Guo,H Li, Z Liu, S Liu. Deoxyaconitine of Metabolite of Aconitine by Human Intestinal Bacteria and Electrospray Ionization Tandem Mass Spectrometry.Chem. J. Chinese U.2007, 28, 2051-2055.
  31. H Dong, Z Liu, F Song, Z Yu,H Li, S Liu. Structural Analysis of Monoterpene Glycosides Extracted fromPaeonia lactiflora Pall.Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and High-performance Liquid Chromatography/ Electrospray Ionization Tandem Mass Spectrometry.Rapid Commun. Mass Spectrom. 2007, 21, 3193-3199.
  32. S Wang,H Li, Z Liu, S Liu, B Cui. Studies on the Contents of Catechin and Epicatechin in Catechu by HPLC,Lishizhen Medicine and Materia Medica Research.2006, 17, 490-491.
  33. H Dong, Z Liu, F Song, Z Yu,H Li, S Liu. Studies on Paeoniflorin by Electrospray Ionization Tandem Mass Spectrometry.Chem. J. Chinese U.2006, 27, 2066-2069.

獲獎記錄

  • AOMSC Young Scientist Travel Award (2017)
  • ASMS Postdoctoral Career Development Award (2014)
  • 2009-2012 Warwick Postgraduate Research Scholarship (WPRS) (2009-2012)
  • University of Warwick Departmental Studentships (2009-2012)
  • Travel Award for the 25thLake Louise Tandem Mass Spectrometry Workshop (2012)
  • IMSF Travel Grant for the 19thInternational Mass Spectrometry Conference (2012)
  • BMSS Travel Award for the 59thASMS Conference on Mass Spectrometry (2011)
  • Travel Award for the 8thUppsala Conference on Electron Capture and Transfer Dissociation (2011)

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