Kiryl Piatkevich進入白俄羅斯國立大學Lyceum開始學習化學專業。從Lyceum畢業後，Piatkevich博士繼續在羅蒙諾索夫莫斯科國立大學攻讀碩士學位，同時從事有機金屬、分析化學、物理化學和生物有機化學等研究。從2019年開始，Piatkevich博士加入西湖大學。
基本介紹
- 中文名：科瑞歐
- 外文名：Kiryl D. Piatkevich
- 國籍：白俄羅斯
人物經歷,研究方向,代表論文,
人物經歷
從幼年開始，Piatkevich博士就對化學產生了極大的興趣。出於對科學的熱情，他15歲便已進入白俄羅斯國立大學Lyceum開始學習化學專業。從Lyceum畢業後，Piatkevich博士繼續在羅蒙諾索夫莫斯科國立大學攻讀碩士學位，同時從事有機金屬、分析化學、物理化學和生物有機化學等研究。在獲得榮譽理學碩士學位後，Piatkevich博士成功獲得繼續攻讀莫斯科國立大學與紐約阿爾伯特愛因斯坦醫學院聯合培養博士的機會，致力於開發小型哺乳動物活體成像的創新方法。
獲得化學博士學位後，Piatkevich博士作為博士後研究員加入麻省理工學院光遺傳學和神經工程學先驅Edward Boyden博士領導的合成神經生物學實驗室。在此期間，Piatkevich博士致力於利用合成生物學研發神經接口的新方法，並研發出了幾種新的分子技術用於觀察活體動物的神經元活動。Piatkevich博士是第一個在主要神經科學模型生物（包括蠕蟲、魚和小鼠）中以高時間精度進行神經元活動的光學記錄的科學家。從2019年開始，Piatkevich博士在西湖大學生命科學學院獨立領導分子生物工程實驗室。
研究方向
Piatkevich博士的研究方向是研發和設計尖端分子和成像技術，用於分析、控制和修復複雜生物系統，如大腦。設計用於光學記錄和操控神經元活動的遺傳編碼分子工具，以及用於神經元解剖學追蹤的螢光探針。這是一個跨學科研究計畫，涵蓋合成和化學生物學、生物工程、生物化學、生物技術、分子體內成像、神經科學、機器人和細胞生物學等領域。Piatkevich實驗室將系統地套用其研發的技術來揭示腦紊亂的分子機制並揭示神經編碼的基本原理。新的分子技術也會用於通過光創建先進的腦機接口。
代表論文
1.Piatkevich K.D.#, Bensussen S.#, Tseng H.#, Shroff S.N., Lopez-Huerta V.G., Park D., Jung E.E., Shemesh O.A., Straub C., Gritton H.J., Romano M.F, Costa E., Sabatini B.L., Fu Z, Boyden E.S., Han X. Population imaging of neural activity in awake behaving mice.Nature 2019,in press(# equal contribution).
2. Qian Y.#,Piatkevich K.D.#, McLarney B.#, Abdelfattah A.S., Mehta S., Gottschalk S., Molina R.S., Zhang W., Drobizhev M., Hughes T.E., Zhang J., Schreiter E.R., Shoham S., Razansky D., Boyden E.S., Campbell R.E. A Genetically Encoded Near-Infrared Fluorescent Calcium Ion Indicator.Nature Methods, 16 (2), 171 (# equal contribution).
3.Piatkevich K.D.#, Jung E.E.#, Straub C., Linghu C., Park D., Suk H.J., Hochbaum D.R., Goodwin D., Pnevmatikakis E., Pak N., Kawashima T., Yang C.-T., Rhoades J.L., Shemesh O., Asano S., Yoon Y.-G., Freifeld L., Saulnier J., Riegler C., Engert F., Hughes T., Drobizhev M., Szabo B., Ahrens M.B., Flavell S.W., Sabatini B.L., Boyden E.S. A Robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.Nature Chemical Biology, 14 (4), 352 (# equal contribution).
4.Piatkevich K.D., Suk H.J., Suhasa B.K., Yoshida F., DeGennaro E.M., Dro bizhev M., Hughes T.E., Desimone R., Boyden E.S.*, Verkhusha V.V. Near-infrared fluorescence proteins engineered from bacterial phytochromes in neuroimaging.Biophys. J. 113(10), 2299-2309.
5.Piatkevich K.D., English B.P., Malashkevich V.N., Xiao H., Almo S.C., Singer R.H., Verkhusha V.V. Photoswitchable red fluorescent protein with a large Stokes shift.Chem Biol.2014. 21:1402.
6.Piatkevich K.D.*, Murdock M.H., Subach F.V.* Advances in engineering and application of optogenetic indicators for neuroscience.Applied Sciences. 2019, 9(3), 562. (*co-corresponding).
7. Tillberg P.W.#, Chen F.#,Piatkevich K.D., Zhao Y., Yu C.C., English B.P., Gao L., Martorell A., Suk H.J., Yoshida F., DeGennaro E.M., Roossien D.H., Gong G., Seneviratne U., Tannenbaum S.R., Desimone R., Cai D., Boyden E.S. Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies.Nature Biotechnology. 34 (9), 987-992 (# equal contribution).
8. Shemesh O.A., Tanese D., Zampini V., Linghu C.,Piatkevich K., Ronzitti E., Papagiakoumou E., Boyden E.S., Emiliani V. Temporally precise single-cell resolution optogenetics.Nature Neuroscience. 20(12), 1796-1806.
9. Subach O.M, Barykina N.V., Anokhin K.V.,Piatkevich K.D., Subach F.V. Near-Infrared Genetically Encoded Positive Calcium Indicator Based on GAF-FP Bacterial Phytochrome.Int. J. Mol. Sci., 20(14), 3488.
10. Subach O.M., Kunitsyna T.A., Mineyeva O.A., Lazutkin A.A., Bezryadnov D.V., Barykina N.V.,Piatkevich K.D., Ermakova Y.G., Bilan D.S., Belousov V.V., Anokhin K.V., EnikolopovG.N.*, Subach F.V.* Slowly reducible genetically encoded green fluorescent indicator for in vivo and ex vivo visualization of hydrogen peroxide.Int. J. Mol. Sci.20(13), 3138;
11. Barykina N.V., Doronin D.A., Subach O.M., Sotskov V.P., Plusnin V.V., Ivleva O.A., Gruzdeva A.M., Kunitsyna T.A., Ivashkina O.I., Lazutkin A.A., Malyshev A.Y., Smirnov I.V., Varizhuk A.M., Pozmogova G.E.,Piatkevich K.D., Anokhin K.V., Enikolopov G.N., Subach F.V*. NTnC-like genetically encoded calcium indicator with a positive and enhanced response and fast kinetics.Scientific Reports, 8 (1), 15233.
12. Doronin D., Barykina N., Subach O., Isaakova E., Varizhuk A., Pozmogova G.,Piatkevich K., Anokhin K., Enikolopov G., Subach F.* Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast.BMC Biotechnology, 18 (1), 10.
13. Barykina N.V., Subach O.M.,Piatkevich K.D., Jung E.E., Malyshev A.Y., Smirnov I.V., Bogorodskiy A.O., Borshchevskiy V.I., Varizhuk A.M., Pozmogova G.E., Boyden E.S., Anokhin K.V., Enikolopov G.N., Subach F.V.*. Green fluorescent genetically encoded calcium indicator based on calmodulin/M13-peptide from fungi.PLoS One.12(8):e0183757.
14. Rowlands C.J., Park D., Bruns O.T.,Piatkevich K.D., Fukumura D., Jain R.K., Bawendi M.G., Boyden E.S., So P.T.C.*. Wide-field three-photon excitation in biological samples.Light: Science & Applications. 6, e16255.
15. Barykina N.V., Subach O.M., Doronin D.A., Sotskov V.P., Roshchina M.A., Kunitsyna T.A., Malyshev A.Y., Smirnov I.V., Azieva A.M., Sokolov I.S.,Piatkevich K.D., Burtsev M.S., Varizhuk A.M., Pozmogova G.E., Anokhin K.V., Subach F.V.*, Enikolopov G.N.*. A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites.Scientific Reports.6:34447.
16. Konold P.E., Yoon E., Lee J., Allen S.L., Chapagain P.P., Gerstman B.S., Regmi C.K.,Piatkevich K.D., Verkhusha V.V., Joo T., Jimenez R.*. Fluorescence from multiple chromophore hydrogen-bonding states in the far-red protein TagRFP675.J. Phys. Chem. Lett.7, 3046-3051.
