連佳長

連佳長，男，畢業於伊利諾伊大學香檳分校，博士，浙江大學博士生導師、百人計畫研究員。

基本介紹

• 中文名：連佳長
• 學位/學歷：博士
• 職業：教師
• 專業方向：· 合成生物學· 代謝工程· 基因組編輯
• 職務：博士生導師
• 職稱：研究員

個人經歷

教育背景

2003-2007，浙江大學，生物工程，學士

2007-2010，浙江大學，生物化工，碩士

2010-2015，伊利諾伊大學香檳分校，化學工程，博士

工作經歷

2015-2017，伊利諾伊大學香檳分校，博士後

2017-至今，浙江大學，化學工程與生物工程學院，“百人計畫”研究員，博導

入選浙江大學“百人計畫”、浙江省海外高層次人才計畫，

受邀擔任BMC Biotechnology副主編(Associate Editor)、Frontiers in Biotechnology and Bioengineeringrs in Biotechnology and Bioengineering客座編輯以及Nature Chemical Biology, Metabolic Engineering, Applied Microbiology and Biotechnology, Biotechnology for Biofuels, Microbial Cell Factories, Journal of Industrial Microbiology and Biotechnology, Enzyme and Microbial Technology, Biochemical Engineering Journal, BMC Biotechnology等期刊審稿人。

研究方向

· 合成生物學

· 代謝工程

· 基因組編輯

主要從事基於合成生物學原理和基因組編輯技術的人工細胞工廠創建研究。

主講課程

基因工程選論（2019，主講）

生物化工前沿（2017，2018，主講老師：姚善涇）

合成生物學導論（2018，主講老師：葉麗丹）

學術成果

研究成果

Lian J#, Schultz C#, Cao M#, HamediRad M#, Zhao H*. Multi-functional Genome-wide CRISPR System for High Throughput Genotype-Phenotype Mapping. Nat Commun, Accepted (#Equal Contribution)

HamediRad M, Chao R, Weisberg S, Lian J, Zhao H*. Towards a fully automated algorithm driven platform for biosystems design. Nat Commun, in press

Liu W, Tang D, Wang H, Lian J*, Huang L, Xu Z* (2019) Combined Genome Editing and Transcriptional Repression for Metabolic Pathway Engineering in Corynebacterium glutamicum using a Catalytically Active Cas12a. Appl Microbiol Biotechnol, in press

Zhao G, Yao S, Rothchild KW, Liu T, Liu Y, Lian J, He HY, Ryan KS, Du YL* (2019) The Biosynthetic Gene Cluster of the C-nucleoside Antibiotic Pyrazomycin with a Rare Pyrazole Moiety. Chembiochem, in press

Liu W, Tang D, Shi R, Lian J*, Huang L, Cai J, Xu Z* (2019) Efficient Production of S-Adenosyl-L-Methionine from DL-Methionine in Metabolic Engineered Saccharomyces cerevisiae. Biotechnol Bioeng, in press

Liu W, Zhu X, Lian J, Huang L, Xu Z* (2019) Efficient Production of Glutathione with Multi-pathway Engineering in Corynebacterium glutamicum. J Ind Microbiol Biotechnol, in press

Jin B, Zhang G, Lian J, Zhang Q, Zhan X, Chen, F. Boron nitride nanosheet embedded bio-inspired wet adhesives with switchable adhesion and oxidation resistance. Journal of Materials Chemistry A, 7 (19), 12266-12275

Xiao F, Wang H, Shi Z, Huang Q, Huang L, Lian J*, Cai J, Xu Z*. Multi-level Metabolic Engineering of Pseudomonas mutabilis ATCC31014 for Efficient Production of Biotin. Metab Eng, in press

Gu Y, Gao J, Cao M, Dong C, Lian J*, Huang L, Cai J, Xu Z*. Construction of a Series of Episomal Plasmids and their Application in the Development of an Efficient CRISPR/Cas9 System in Pichia pastoris. World J Microbiol Biotechnol, 35 (6): 79

HamediRad M, Weisberg S, Chao R, Lian J, Zhao H*. Highly Efficient Single-Pot Scarless Golden Gate Assembly. ACS Synth Biol, in press

Jiang R, Chen X, Lian J*, Huang L, Cai J, Xu Z* (2019) Efficient Production of Pseudoionone with Multi‐pathway Engineering in Escherichia coli. J Appl Microbiol, 126 (6): 1751-1760

Wang H, Liu W, Shi F, Huang L, Lian J*, Qu L, Cai J, Xu Z* (2018) Metabolic Pathway Engineering for High-level Production of 5-Hydroxytryptophan in Escherichia coli. Metab Eng, 48: 279-287

Lian J, Mishra S, Zhao H* (2018) Recent Advances in Metabolic Engineering of Saccharomyces cerevisiae: New Tools and their Applications. Metab Eng, 50: 85-108

Schultz C, Lian J, Zhao H. Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion. Methods Enzymol, 608: 265-276

Jiang L, Zhao J, Lian J*, Xu Z* (2018) Cell-Free Protein Synthesis Enabled Rapid Prototyping for Metabolic Engineering and Synthetic Biology. Synth Syst Biotechnol, 3: 90-96

Wei P, Zhuang B, Yu D, Sharipova A, Cai J, Huang L, Lian J*, Xu Z* (2018) Cell-Free Expression of Unnatural Amino Acid Incorporated Aquaporin SS9 with Improved Separation Performance in Biomimetic Membranes. BioMed Res Int

Lian J, HamediRad M, Zhao H* (2018) Advancing Metabolic Engineering of Saccharomyces cerevisiae using the CRISPR/Cas System. Biotechnol J, 13 (9): 1700601

HamediRad M, Lian J, Li H, Zhao H* (2018) RNAi Assisted Genome Evolution Unveils Yeast Mutants with Superior Xylose Utilization. Biotechnol Bioeng, 115: 1552-1560

Lian J, Bao Z, Hu S, Zhao H* (2018) Engineered CRISPR/Cas9 System for Multiplex Genome Engineering of Polyploid Industrial Yeast Strains. Biotechnol Bioeng, 115: 1630-1635

Lian J, HamediRad M, Hu S, Zhao H* (2017) Combinatorial Metabolic Engineering using an Orthogonal Tri-functional CRISPR System. Nat Commun 8 (1), 1688

Si T#, Lian J#, Zhao H* (2017) Strain Development by Whole-Cell Directed Evolution. Directed Enzyme Evolution: Advances and Applications (Alcalde M, Ed) pp:173–200, Springer International Publishing (#Equal Contribution)

Lian J, Jin R, Zhao H* (2016) Construction of Plasmids with Tunable Copy Numbers in Saccharomyces cerevisiae and their Applications in Pathway Optimization and Multiplex Genome Integration. Biotechnol Bioeng 113: 2462–2473

Lian J, Zhao H* (2016) Functional Reconstitution of a Pyruvate Dehydrogenase in the Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering. ACS Synt Biol 5: 689–697

Coursolle D, Lian J, Zhao H* (2015) Production of Long Chain Alcohols and Alkanes Upon Coexpression of an Acyl-ACP Reductase and Aldehyde-Deformylating Oxygenase with a Bacterial Type-I Fatty Acid Synthase in E. coli. Mol BioSyst 11: 2464-2472

Lian J, Zhao H* (2015) Recent Advances in Biosynthesis of Fatty Acids Derived Products in Saccharomyces cerevisiae via Enhanced Supply of Precursor Metabolites. J Ind Microbiol Biotechnol 42: 437-451

Lian J, Zhao H* (2015) Reversal of the β-Oxidation Cycle in Saccharomyces cerevisiae for Production of Fuels and Chemicals. ACS Synt Biol 4: 332-341

Feng X, Lian J, Zhao H* (2015) Metabolic Engineering of Saccharomyces cerevisiae to Improve 1-Hexadecanol Production. Metab Eng 27: 10-19

Lian J, Si T, Nair NU, Zhao H* (2014) Design and Construction of Acetyl-CoA Overproducing Saccharomyces cerevisiae Strains. Metab Eng 24: 139-149

Lian J, Li Y, Hamedi Rad H, Zhao H* (2014) Directed Evolution of a Cellodextrin Transporter for Improved Biofuel Production under Anaerobic Conditions in Saccharomyces cerevisiae. Biotechnol Bioeng 111: 1521-1531

Lian J, Chao R, Zhao H* (2014) Metabolic Engineering of a Saccharomyces cerevisiae Strain Capable of Simultaneously Utilizing Glucose and Galactose to Produce Enantiopure (2R,3R)-Butanediol. Metab Eng 23: 92-99

Zhang X, Lian J, Kai L, Huang L, Cen P, Xu Z* (2014) Enhanced Functional Expression of Aquaporin Z via Fusion of in situ Cleavable Leader Peptides in Escherichia coli Cell-free System. Enyme Microb Technol 55: 26-30

Eriksen D#, Lian J#, Zhao H* (2014) Protein Design for Pathway Engineering. J Struct Biol 185(2): 234-242 (#Equal Contribution)

Du J, Yuan Y, Si T, Lian J, Zhao H* (2012) Customized Optimization of Metabolic Pathways by Combinatorial Transcriptional Engineering. Nucleic Acids Res 40(18): e142

Kai L, Kaldenhoff R, Lian J, Zhu X, Dotsch V, Bernhard F, Cen P, Xu Z* (2010) Preparative Scale Production of Functional Mouse Aquaporin 4 Using Different Cell-free Expression Modes. PLoS ONE 5(9): e12972

Zhou J, Huang L, Lian J, Sheng J, Cai J, Xu Z* (2010) Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system. Biotechnol Lett 32:1481-1486

Zhang D, Wei P, Fan L, Lian J, Huang L, Cai J, Xu Z* (2010) High-level Soluble Expression of hIGF-1 Fusion Protein in Recombinant Escherichia coli. Process Biochem 45(8): 1401-1405

Xu Z*, Lian J, Cai J (2010) Efficient Expression of Aquaporin Z in Escherichia coli Cell-free System Using Different Fusion Vectors. Protein Pept Lett 17(2): 181-185

Lian J, Ma Y, Cai J, Wu M, Wang J, Wang X, Xu Z* (2009) High-level Expression of Soluble Subunit b of F1F0-ATP Synthase in Escherichia coli Cell-free System. Appl Microbiol Biotechnol 85(2): 303-311

Lian J, Ding S, Cai J, Zhang D, Xu Z*, Wang X (2009) Improving Aquaporin Z Expression in Escherichia coli by Fusion Partners and Subsequent Condition Optimization. Appl Microbiol Biotechnol 82(3): 463-470

Lian J, Fang X, Cai J, Chen Q, Zheng Q, Kai L, Xu Z* (2008) Efficient Expression of Membrane-Bound Water Channel Protein (Aquaporin Z) in Escherichia coli. Protein Pept Lett 15(7): 687-691

科研項目

基於合成生物學原理和基因組編輯技術的人工細胞工廠創建研究

榮譽獎項

浙江省傑出青年基金獲得者。