蔡謹，男，副教授，浙江大學碩士生導師，工學博士。
基本介紹
- 中文名：蔡謹
- 畢業院校：浙江大學
- 學位/學歷：博士
- 專業方向：生物化工
- 就職院校：浙江大學
個人經歷,研究方向,出版圖書,主講課程,學術成果,主持項目,論著,論文,
個人經歷
1984年於杭州大學生物系本科畢業。1984年-1988年於浙江醫科大學任教。1988年-1991年浙江大學生物科學與技術系攻讀碩士學位。1991年至今在浙江大學化學工程系任教。2002年~2003年澳大利亞墨爾本大學訪問學者。
研究方向
（1）無細胞體外高效合成蛋白質新技術；
（2）新型蛋白質修飾及酶固定化技術；
（3）重要工業微生物的代謝工程
出版圖書
作者類型：
作者時間：2002年12月01日
《生命的催化劑酶工程》是2002年浙江大學出版社出版的圖書，作者是蔡謹，孟文芳等。
展開
主講課程
本科生專業核心課程《工業微生物學》
研究生選修課程《細胞工程》和必修課程《微生物工程》
工程碩士學位課程《生物製藥選論》
學術成果
主持項目
近年主持國家自然基金面上項目兩項。主持國家863支撐項目1項。作為浙大方負責人主持國家863項目2項。主持4項省級項目和橫向課題。參加國家級項目和省級項目若干項。
【1】國家自然科學基金項目：“深海嗜壓菌水通道蛋白基因的高效表達和功能特性研究；“重組水通道蛋白在模擬生物膜中的整合及其功能重建”。
【2】863計畫項目：“非糧原料厭氧發酵高產丁酸的關鍵技術和新工藝”；“生物聚合物材料的全生物合成技術”；“能源和醫藥產品模組化設計合成”。
論著
[1]岑沛霖，蔡謹《工業微生物學》化工出版社2008（十一五、十二五國家級規劃教材）
[2]蔡謹主譯《微生物學：原理與探索》中譯本化學工業出版社2007
[3]蔡謹，柴紅，岑沛霖《工業微生物學多媒體網路課件》（CD-ROM）化學工業出版社2003年
[4]蔡謹《生命的催化劑：酶工程》浙江大學出版社 2002
論文
[1] X Zhang, JY Sheng, L Huang, LP Du, J Cai, PL Cen, ZN Xu. (2014) High-level soluble expression of one model olfactory receptor (ODR-10) in Escherichia coli cell-free system. World J. Microbiol. Biotechnol., 30(3): 893-901.
[2].JY Sheng, L Huang, XC Zhu, J Cai, ZN Xu. (2014) Reconstitution of the peptidoglycan cytoplasmic precursor biosynthetic pathway in cell-free system and rapid screening of antisense oligonucleotides for Mur enzymes. Appl. Microbiol. Biotechnol., 98(4): 1785-1794.
[3]F Zhu, J Cai, XT Wu, J Huang, L Huang, JZ Zhu, Q Zheng, PL Cen, ZN Xu. (2013) The main byproducts and metabolic flux profiling of gamma-PGA-producing strain B. subtilis ZJU-7 under different pH values. J Biotechnol. 164(1): 67-74.
[4].W Luo, J Huang, XC Zhu, L Huang, J Cai, ZN Xu. (2013) Enhanced Production of L-Tryptophan with Glucose Feeding and Surfactant Addition and Related Metabolic Flux Redistribution in the Recombinant Escherichia coli. Food Sci. Biotechnol. 22(1): 207-214.
[5].J Huang, S Jiang, XQ Xu, HS Wu, XC Zhu, ZM Ke, J Cai, L Huang, ZN Xu.(2012). Effects of carbon/nitrogen ratio, dissolved oxygen and impeller type on gellan gum production in Sphingomonas paucimobilis. Ann. Microbiol. 62(1): 299-305.
[6].L Huang, YJ Xiang, J Cai, L Jiang, ZB Lv, YZ Zhang, ZN Xu*. (2011) Effects of three main sugars in cane molasses on the production of butyric acid with Clostridium tyrobutyricum. Korean J. Chem. Eng. 28(12): 2312-2315.
[7].Z Yang, RR Ma, L Huang, XC Zhu, JY Sheng, J Cai, W Hu, ZN Xu.(2012) High-level production of soluble adenine nucleotide translocator from Schistosoma japonicum in E. coli cell-free system. Process Biochem. 47(3):395-400.
[8].WM Gao, XC Zhu, J Cai, L Huang, PL Cen, ZN Xu.(2012) Refolding and two-step purification by hydrophobic interaction chromatography of recombinant human bone morphogenetic protein-2 from Escherichia coli. Process Biochem. 47(6):960-967.
[9].YP Liu, JF Pan, PL Wei, JZ Zhu, L Huang, J Cai, ZN Xu(2012) Efficient expression and purification of recombinant alcohol oxidase in Pichia pastoris. Biotechnol. Bioprocess Eng. 17(4):693-702.
[10].XC Zhu, Q Zheng, H Yang, J Cai1, L Huang, YW Duan,ZN Xu, PL Cen.(2012) Recent advances in inkjet dispensing technologies: applications in drug discovery. Expert Opin. Drug Discov.. 7(9):761-770.
[11].Y Yu, PL Wei, XC Zhu, L Huang, J Cai, ZN Xu.(2012) High-level production of soluble pyrroloquinoline quinone -dependent glucose dehydrogenase in Escherichia coli. Eng. Life Sci. 12(5):1-9.
[12].W Luo, J Cai, LQ Zhu, XC Zhu, L Huang, ZN Xu, PL Cen.(2012) Toxic effects of acrylic acid on Clostridium propionicum and isolation of acrylic acid-tolerant mutants for potential production of acrylic acid. Eng. Life Sci. 12(5):567-573.
[13].ZM Shi, PL Wei, XC Zhu, J Cai, L Huang, ZN Xu.(2012) Efficient production of L-lactic acid from hydrolysate of Jerusalem artichoke with immobilized cells of Lactococcus lactis in fibrous bed bioreactors. Enzyme Microb. Technol. 51(5):263-268.
[14].HL Zhang, JZ Zhu, XC Zhu, J Cai, AY Zhang, YZ Hong, J Huang, L Huang, ZN Xu: High-level exogenous glutamic acid-independent production of poly-(c-glutamic acid) with organic acid addition in a new isolated Bacillus subtilis C10. Bioresour Technol. 116: 241-246.
[15].Q Zheng, R Shi, XC Zhu, L Huang, J Cai, WB Han, ZN Xu.(2012) Functional expression of Bacillus subtilis xylanase A in an Escherichia coli derived cell-free protein synthesis system and subsequent expression improvement via DNA gel technique. Process Biochem. 116: 241-246.
[16].LQ Zhu, PL Wei, J Cai, XC Zhu, ZM Wang, L Huang, ZN Xu.(2012) Improving the productivity of propionic acid with FBB-immobilized cells of an adapted acid-tolerant Propionibacterium acidipropionici. Bioresour Technol. 11(3):238-244.
[17].J Huang, J Cai, J Wang, XC Zhu, L Huang, ST Yang, ZN Xu.(2011) Efficient production of butyric acid from Jerusalem artichoke by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor. Bioresour Technol., 108(1):31-40.
[18].Q Zheng, JG Lu, H Chen, L Huang, J Cai, ZN Xu. (2011) Application of inkjet printing technique for biological material delivery and antimicrobial assays. Anal Biochem. 410(2): 171-178.
[19].CL Shu, J Cai, L Huang, XC Zhu, ZN Xu.(2011) Biocatalytic production of ethyl butyrate from butyric acid with immobilized Candida rugosa lipase on cotton cloth. J. Mol. Catal. B-Enzym. 72(3-4): 139-144.
[20].HL Zhang, J Cai, JQ Dong, DP Zhang, L Huang, ZN Xu, PL Cen. (2011) High-level production of poly (beta-L-malic acid) with a new isolated Aureobasidium pullulans strain. Appl. Microbiol. Biotechnol.. 2011, 92(2): 295-303.
[21].LQ Zhu, H Chen, L Huang, J Cai, ZN Xu. (2011) Electrochemical analysis of Clostridium propionicum and its acrylic acid production in microbial fuel cells. Eng. Life Sci.11(3):238-244.
[22].W Luo, YP Liu, XC Zhu, WJ Zhao, L Huang, J Cai, ZN Xu, PL Cen.(2011)Cloning and characterization of purine nucleoside phosphorylase in Escherichia coli and subsequent ribavirin biosynthesis using immobilized recombinant cells. Enzyme Microb. Technol. 48(6-7): 438-444.
[23]RR Ma, Z Yang, L Huang, XC Zhu, L Kai, J Cai, XN Wang, ZN Xu. (2011) Construction of an efficient Escherichia coli cell-free system for in vitro expression of several kinds of proteins. (2010) Eng. Life Sci.. 10(4): 333-338.
[24].JW Zhou, L Huang, JZ Lian, JY Sheng, J Cai, ZN Xu. (2010) Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system. Biotechnol Lett. 32(10): 1481-1486.
[25].JW Zhou, LM Fan, PL Wei, L Huang, J Cai, ZN Xu. (2010) Efficient production of Uridine 5'-Diphospho-N-Acetylglucosamine by the combination of three recombinant enzymes and yeast cells. Prep. Biochem. Biotechnol.. 40(4): 294-304.
[26].DP Zhang, PL Wei, LM Fan, JZ Lian, L Huang, J Cai, ZN Xu. (2010) High-level soluble expression of hIGF-1 fusion protein in recombinant Escherichia coli. Process Biochem., 45(8): 1401-1405.
[27].MH Wang, J Cai, L Huang, ZB Lv, YZ Zhang, ZN Xu. (2010) High-level expression and efficient purification of bioactive swollenin in Aspergillus oryzae. Appl. Biochem. Biotechnol. , 162 (7): 2027-2036.
