Subtractive hybridization是以PCR增幅為基礎的技術。
基本介紹
- 中文名:減差雜交
- 外文名:Subtractive hybridization
- )試劑盒:PCR-select cDNA Subtraction Kit (Clontech
- 技術:以PCR增幅為基礎的


Subtractive hybridization是以PCR增幅為基礎的技術。


扣除雜交(Subtractive Hybridization)是通過構建扣除文庫分離差異表達基因的分子生物學技術。其核心原理是利用不同來源核酸的互補性差異,通過過量雜交去除共有序列,保留目標單鏈核酸片段進行克隆富集,適用於低豐度基因篩選及突變基因分離。典型套用包括T細胞受體基因分離、植物突變區域DNA富集及藥物靶基因篩選。該技術最早...
抑制性減法雜交suppression subtractive hybridization,ssh 1996年,L.Diathebko在RDA的基礎上建立了抑制性減法雜交技術,該技術是RDA技術的發展,能有效克服RDA或cDNA RDA技術難以解決的問題。主要原理 其中標準化步驟均等了檢測子中的cDNA單鏈豐度,而消減雜交步驟去除了檢測子和驅趕子之間的共同序列,使檢測子和驅趕...
抑制性消減雜交(suppression subtractive hybridization, SSH)技術是一種鑑定、分離組織細胞中選擇性表達基因的技術,其原理是以抑制性多聚酶鏈反應(PCR)反應為基礎的cDNA 消減雜交技術。簡介 通過合成兩個不同的接頭,連線於測試cDNA 片段的5’末端,達到選擇性擴增差異性表達的cDNA 片段,抑制非目的cDNA的擴增。該技術...
抑制差減雜交( suppression subtractive hybridization,SSH)是基於抑制PCR和差減雜交技術建立的簡單有效的方法[1],通過一次差減雜交可使低豐度的序列(mRNA)得以高於1000倍的富集,有效的選擇性擴增目標序列,同時抑制非目標序列的擴增,因此在分離基因特別是分離低豐度差異表達基因方面具有更廣闊的套用前景。在轉錄水平上...
抑制消減雜交(suppression subtractive hybridization,SSH) 是建立在抑制PCR 與消運用雜交二級動力學原理,減雜交技術相結合的基礎上的更簡單快速的分離差異基因的方法。即豐度高的單鏈DNA 在退火時產生同源雜交的速度快於豐度低的單鏈DNA,使原來在豐度上有差別的單鏈DNA 相對含量達到基本一致,利用鏈內退火優於鏈間...
RSDD,reciprocal subt raction differentialRNAdisplay,互動式差減差異RNA顯示技術,與代表性差式分析(representative difference analysis, RDA),抑制性差減雜交( suppression subtractive hybridization, SSH)一樣,是一種較新的差異表達基因的分離技術。RSSD可以有效快速地鑑定由於細胞生理變化而在基因組中差異表達的...
它主要基於最近出現的抑制PCR , 並結合標準化(normalization 或equalization ) 和消減雜交( substractive hybridization)。抑制PCR 通過利用含引物序列的雙連結頭(adaptor) 充當引物模板, 使兩端接上同一接頭的非目的雙鏈片段具有反向末端重複序列, PCR 中不能擴增, 從而達到抑制非目的片段而選擇性擴增目的片段的目的。...
(29)An, X.P., Hou, J.X., Li, G., Peng, J.Y., Liu, X.Q., Liu, H.Y., Xiao, W.P., Wang, J.G., Song, Y.X., Cao, B.Y., 2012a. Analysis of Differentially Expressed Genes in Ovaries of Polytocous versus Monotocous Dairy Goats Using Suppressive Subtractive Hybridizatio...
20 Retrieval of Full-Length Functional Genes Using Subtractive Hybridization Magnetic Bead Capture Tracy Meiring,Inonge Mulako,Marla I.Tuffin,Quinton Meyer,and Donald A.Cowan 21 Detection and Isolation of Selec
Xiaoli Shi, Mingyu Shao, Litao Zhang, Yubin Ma, Zhifeng Zhang. Screening of genes related to sulfide metabolism in Urechis unicinctus (Echiura, Urechidae) using suppression subtractive hybridization and cDNA microarray analysis. Comparative Biochemistry and Physiology- Part D: Genomics and Proteomics, ...
《牛筋草(Eleusine indica)對百草枯抗性水平差異分子遺傳機理研究》是依託華南農業大學,由陳勇擔任項目負責人的面上項目。項目摘要 本項目以我國抗性和敏感型牛筋草(Eleusine indica)種群為材料,通過SSH(suppression subtractive hybridization)方法,分析比較牛筋草抗性植株和敏感植株mRNA的表達差異,構建...
34.Luo D,Hu W, Chen S, Xiao Y, Sun Y, Zhu Z. Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization. Biology of Reproduction, 2009,80:674-684.35. Guan B,Hu W, ...
10. Mei Zhang, Xuncheng Liu, Lianyu Yuan, Keqiang Wu,Jun Duan, Xiaolan Wang, Lixiang Yang. Transcriptional profiling in cadmium-treated rice seedling roots using suppressive subtractive hybridization. Plant Physiology and Biochemistry. 50 (2012):79-86.11. Kunlin Wua, Songjun Zenga, Jaime A....
H.Y. Wang, Y.H. Hu, Y. Liu, Y.T. Zhou, M.L. Wang and Y. Zhao*. Suppression subtractive hybridization identifies differentially expressed genes in Brassica napus chlorophyll-reduced mutant. Biologia Plantarum. 2008, 52 (3): 486-492 J Li, S Song,* X Liu, L Wang, D Pan, Q Huang...
[42]Guo Wei-Li, Chen Ru-Gang, Gong Zhen-Hui*, Yin Yan-Xu, Li Da-Wei, Suppression subtractive hybridization analysis of genes regulated by application of exogenous abscisic acid in pepper plant (Capsicum annuum L.) leaves under chilling stress, Plos One, 2013, 8(6):e66667 [43]Zhang Y-...
63. Li, N., Zhang, J., Zhang, L.Q. & Nie, P. (2010). Difference in genes between a high virulence strain G4 and a low virulence strain G18 of Flavobacterium columnare by using suppression subtractive hybridization. Journal of Fish Diseases 33: 403-412.64. Li, W.X., Wang, G.T...
30.Screening for metronidazole-resistance associated gene fragments of H pylori by suppression subtractive hybridizationWJG 2007,March 28;13(12):1847-1850 第二。31.Gynura root induces hepatic veno-occlusive disease: a case report and review of the literatureWJG 2007 March 14; 13(10): 1628-...
第21章 細胞衰老研究中的消減雜交技術 Subtractive Hybridization Techniques to Study Cellular Senescence Michal Bassani Sternberg,Shimon Gepstein 第22章 定位影響果蠅壽命自然差異的遺傳多態性 Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity Maria De Luca,Jeff Le 第23章 在老化系統...
該基因是我室在前期研究中利用cDNA微陣列技術聯合抑制性消減雜交(suppression-subtractive hybridization,SSH)分離鑑定的鼻咽部相對特異表達基因。BPIFB1在鼻咽癌中表達顯著下調,且其低表達與鼻咽癌患者的不良預後相關。放射治療是目前鼻咽癌臨床治療中的主要手段,儘管放療可以達到較好的局部控制效果,...
Sciences . 2011; 7: 476-486.9.Luo D, Hu W*, Chen S, Xiao Y, Sun Y, Zhu Z*. Identification of differentially expressed genes between cloned and zygote-developing zebrafish embryos at the dome stage using suppression subtractive hybridization.Biology of Reproduction.2009; 80: 674-684.
7.Xinping Li, Jiang Zhu, Hongqi Huo. A profile of Differentially Expressed Genes in Blood Vessel and Blood Cell of Hyperlipidemia Rats Using Suppression Subtractive Hybridization. Journal of Nanoscience and Nanotechnolog,2005,5:1287–1291.(SCI,1.93)8.李新平,陳曦,王麗娜,李創宏,張文龍,...
12. Zhong XP, Wang D, Zhang YB, Gui JF. 2009. Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization. Comp Biochem Physiol B Biochem Mol Biol. 152(2):161-70.13. Zhao JG, Zhou L, Jin JY, Zhao Z...
3. Zeng FC, Zhang XL, Zhu LF, TuLL, Guo XP and Nie YC. Isolation and characterization of genes associated to cotton somatic embryogenesis by suppression subtractive hybridization and macroarray. Plant Molecular Biology, 2006,60(2): 169-185.4.Zhu Longfu, Zhang Xianlong., Tu Lili., et al....
9. Qiu WM, Zhu AD, Wang Y, Ge XX, Chai LJ, Deng XX, Guo WW* (2012) Comparative transcript profiling of gene expression between seedless Ponkan mandarin and its seedy wild type during floral organ development by suppression subtractive hybridization and cDNA microarray. BMC Genomics 13: 397 ...
Yusu Xie, Fuhua Li*, Bing Wang, Shihao Li, Dongdong Wang, Hao Jiang, Chengsong Zhang, Kuijie Yu, Jianhai Xiang, 2010. Screening of genes related to ovary development in Chinese shrimp Fenneropenaeus chinensis by suppression subtractive hybridization. Comparative Biochemistry and Physiology, Part D...
(5)Guo WL, Chen RG, Gong ZH, Yin YX, Li DW. Suppression subtractive hybridization analysis of genes regulated by application of exogenous abscisic acid in pepper plant (Capsicum annuum L.) leaves under chilling stress. PLoS ONE 2013, 8 (6): e66667. doi:10.1371 /journal. pone.0066667(...
