胡凱鋒，男，1974年02生， 研究員，中國科學院昆明植物研究所 博士生導師，2012年入選“青年千人計畫”
基本介紹
- 中文名：胡凱鋒
- 國籍：中國
- 民族：漢
- 出生日期：1974年02月
- 職業：研究員，博士生導師
個人簡介,教育背景,研究領域,代表論著,
個人簡介
教育背景
1995年 北京醫科大學(現北京大學醫學部), 學士
1998年 中國科學院上海藥物研究所， 碩士
1998.9 -2000.10 美國馬里蘭大學藥學院， 博士生
2004 瑞士聯邦高等理工學院 (ETH)， 博士
2005 - 2008 美國國立衛生研究院（NIH, NIDDK） 博士後
2009 - 2012 美國威斯康星大學(NMRFAM) 助理科學家
2012 - 至今 中國科學院昆明植物研究所 研究員、博士生導師
2012.2 入選“青年千人計畫”
研究領域
1. 基於核磁共振技術的結構生物學：與重大疾病相關或者與重要生命過程相關的蛋白質的空間三維結構；
基於結構生物學的藥物發現；研究靶標蛋白質分子與化學小分子之間的相互作用以及小分子藥物對生物大分子功能的調節作用；
2. 基於結合核磁共振技術和液質聯用技術的代謝組學：發現與重大疾病相關的生物分子標識物；
3. 中藥多組分成分分析，結合核磁共振技術和液質聯用技術，建立基於中藥有效生物活性成分的中藥質量評價體系
4. 核磁共振技術新方法開發與套用
代表論著
1. Kaifeng Hu, Alexander Eletsky and Konstantin Pervushin*, Backbone resonance assignment in large protonated proteins using a combination of new 3D TROSY-HN(CA)HA, 4D TROSY-HACANH and C detected HACACO experiments. (2003) J. Biomol NMR, 26 (1), 66-77.
2. Kaifeng Hu*, Beat Vögeli and Konstantin Pervushin*, Side-chain H and C resonance assignment in protonated / partially deuterated proteins using an improved 3D 13C-detected HCC-TOCSY. (2005) Journal of Magnetic Resonance, 174 (2), 200-208.
3. Kaifeng Hu¶, Beat Vögeli¶ and G. Marius Clore*, Interference between transverse cross-correlated relaxation and longitudinal relaxation affects apparent J-coupling and transverse cross-correlated relaxation. (2006) Chem. Phys. Letter, 423, 123-125
4. Kaifeng Hu*, VeniaminGalius, and Konstantin Pervushin*,Structural plasticity of peptidyl-prolylisomerasesFkpA is a key to its chaperone function as revealed by solution NMR. (2006) Biochemistry,45(39), 11983-11991
5. Kaifeng Hu¶, Beat Vögeli¶ and G. Marius Clore*,13C-detected HN(CA)C and HMCMC experiments using a single methyl-reprotonated sample for unambiguous methyl resonance assignment. (2006) J. Biomol NMR, 36(4), 259-66.
6. Maria U. Johansson*, Simon Alioth, Kaifeng Hu, RetoWalser, Ralf Koebnik and Konstantin Pervushin*, A minimal transmembraneβ-barrel platform protein studied by NMR. (2007) Biochemistry, 46(5), 1128-1140.
7. Kaifeng Hu, Beat Vögeli and G. Marius Clore*, Spin state selective 13C-detected HNCO with TROSY optimization in all dimensions and double echo-antiecho sensitivity enhancement in both indirect dimensions. (2007) J. Am. Chem. Soc., 129(17), 5484-91.
8. Jun Hu¶, Kaifeng Hu¶, David C. Williams Jr., Michal Komlosh, MengliCai and G. Marius Clore*, Solution NMR structures of productive and non-productive complexes between the A and B domains of the cytoplasmic subunit of the mannose transporter of the Escherichia coli phosphotransferase system, (2008) J. Biol. Chem. 283, 11024-11037.
9. Kaifeng Hu, MichaeleenDoucleff and G. Marius Clore*, Using multiple quantum coherence to increase the 15N resolution in a three-dimensional TROSY HNCO experiment for accurate PRE and RDC measurements, (2009) Journal of Magnetic Resonance, 200 (2), 173-177.
10. Zheng Zhou, HanqiaoFeng, Bing-Rui Zhou, Rodolfo Ghirlando, Kaifeng Hu, Adam Zwolak, Lisa M. Jenkins, Hua Xiao, NicoTjandra, Carl Wu, &YawenBai*, Structural basis for recognition of centromere specific histone H3 variant by nonhistone Scm3, (2011) Nature, 472(7342), 234-7.
11. Kaifeng Hu, William M. Westler, and John L. Markley*, Simultaneous quantification and identification of individual chemicals in metabolite mixtures by two-dimensional extrapolated time-zero 1H-13C HSQC (HSQC0), (2011) J. Am. Chem. Soc., 133(6), 1662-5.
12. Kaifeng Hu, William M. Westler and John L. Markley*,Two-dimensional concurrent HMQC-COSY as an approach for small molecule chemical shift assignment and compound identification, (2011) J. Bio. NMR. 49(3-4), 291-6.
13. Roger A. Chylla*, Kaifeng Hu, James J. Ellinger, and John L. Markley, Deconvolution of Two-Dimensional NMR by Fast Maximum Likelihood Reconstruction (FMLR): Application to Quantitative Metabolomics, (2011) Analytical Chemistry. 83(12), 4871-80.
14. Kaifeng Hu, Thomas P. Wyche, Tim S. Bugni and John L. Markley*, Selective Quantification by 2D HSQC0 Spectroscopy of Thiocoraline in an Extract from a Sponge-DerivedVerrucosisporasp., (2011) Journal of Natural Products, 74(10), 2295-8.
15. Kaifeng Hu*, James J. Ellinger, Roger A. Chylla and John L. Markley, Measurement of Absolute Concentrations of Individual Compounds in Metabolite Mixtures by Gradient-Selective Time-Zero 1H-13C HSQC (gsHSQC0) with Two Concentration References and Fast Maximum Likelihood Reconstruction Analysis, (2011) Analytical Chemistry, 83(24), 9352-60.
16. Williard J. Werner, Kylie D. Allen, Kaifeng Hu, Gregory L. Helms, Brian S. Chen, and Susan C Wang*, In vitro Phosphinate Methylation by PhpK from KitasatosporaPhosalacinea, (2011) Biochemistry,50(42), 8986-8.
