《果蠅實驗指南》是2003年科學出版社出版的圖書，作者是W.沙利文。本書共提供了近40個未來10年內最可能用到的以果蠅為實驗對象的分子生物學、生物化學和細胞生物學研究的實驗方案。
基本介紹
- 作者：W.沙利文 編
- ISBN：9787030124401
- 頁數：697
- 定價：90.00元
- 出版社：科學出版社
- 出版時間：2003-12
- 裝幀：簡裝本
內容介紹,目錄,
內容介紹
《果蠅實驗指南(影印版)》簡介：果蠅是遺傳學、發育學研究的重要模式生物之一，對於發育生物學、基因組學領域中的探索具有極為重要的意義。每一個方案都經過專家的精心挑選和雕琢，實驗設計嚴謹、準確、簡潔、規範，可操作性強，值得稱道。方案涉及染色體、細胞、基因組和發育等方面，既包含了初學者需要了解的基礎知識，也涵蓋了資深研究者所需的細節。《果蠅實驗指南(影印版)》的版式設計側重於方便讀者使用，正文中穿插了豐富的圖表作為實驗設計的輔助說明，附錄中還列出了果蠅研究所需的重要資料如基本溶液、緩衝液的配方和配製方法等。
目錄
Preface
Abbreviations
CHROMOSOMES
1 Preparation and Analysis of Drosophila Mitotic Chromosomes
2 In Situ Hybridization to Somatic Chromosomes
3 BrdU Labeling of Chromosomes
4 Analysis of Meiosis of Fixed and Live Oocytes by Light Microscopy
5 Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster
6 Preparation and Analysis of Polytene Chromosomes
7 In Situ Hybridizaiton to Polytene Chromosomes
8 Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
CELL BIOLOGY
9 Fluorescent Analysis of Drosophila Embryos
10 Imaginal Discs
11 The Neuromuscular Junction
12 Histological Techniques for the Drosophila Eye.Part Ⅰ:Larva and Pupa
13 Histological Techniques for the Drosophila Eye.Part Ⅱ:Adult
14 Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy
15 Electrophysiological Approaches to the Neuromusculature
16 Functional Assays of the Peripheral and Central Nervous Systems
17 GFP and Other Reporters
18 Quantitative Microinjection of Drosophila Embryos
19 Targeted Disruption of Gene Function in Drosophila by RNA Interference
20 Drosophila Cell Culture and TRransformation
21 Generating Antibodies against Drosophila Proteins
22 Photoactivated Gene Expression for Cell-fate Mapping and Cell Manipulation
MOLECULAR BIOLOGY
23 Recovery of DNA Sequences Flanking P-element Insertions:Inverse PCR and Plasmid Rescue
24 GAL4-mediated Ectopic Gene Expression in Drosophila
25 Functional Cell Ablation
GENOMICS
26 Preparation of DNA from E.coli Cells Containing Bacteriophage P1 Clones
27 Drosophila Microarrays:From Arrayer Construction to Hybridization
28 Using Drosophila Genome Databases
29 Preparation and Analysis of High-molecular-weight DNA in Drosophila
BIOCHEMISTRY
30 Culturing Large Populations of Drosophila for Protein Biochemistry
31 Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis
32 Preparation of Membrane Proteins from Drosophila Embryos
33 Preparing Cytoplasmic Extracts from Drosophila Embryos
34 Immunoblotting of Proteins from Single Drosophila Embryos
THE ORGANISM
35 Laboratory Culture of Drosophila
36 Preparation of Larval and Adult Cuticles for Light Microscopy
37 Exposing Drosophila to Neuroactive Drugs
APPENDICES
1 1989 Table of Contents from Drosophila:A Laboratory Manual
2 Anatomical Drawings of Drosophila
3 Solutions and Reagents
4 Cautions
5 Suppliers
6 Trademarks
Index
Abbreviations
CHROMOSOMES
1 Preparation and Analysis of Drosophila Mitotic Chromosomes
2 In Situ Hybridization to Somatic Chromosomes
3 BrdU Labeling of Chromosomes
4 Analysis of Meiosis of Fixed and Live Oocytes by Light Microscopy
5 Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster
6 Preparation and Analysis of Polytene Chromosomes
7 In Situ Hybridizaiton to Polytene Chromosomes
8 Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
CELL BIOLOGY
9 Fluorescent Analysis of Drosophila Embryos
10 Imaginal Discs
11 The Neuromuscular Junction
12 Histological Techniques for the Drosophila Eye.Part Ⅰ:Larva and Pupa
13 Histological Techniques for the Drosophila Eye.Part Ⅱ:Adult
14 Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy
15 Electrophysiological Approaches to the Neuromusculature
16 Functional Assays of the Peripheral and Central Nervous Systems
17 GFP and Other Reporters
18 Quantitative Microinjection of Drosophila Embryos
19 Targeted Disruption of Gene Function in Drosophila by RNA Interference
20 Drosophila Cell Culture and TRransformation
21 Generating Antibodies against Drosophila Proteins
22 Photoactivated Gene Expression for Cell-fate Mapping and Cell Manipulation
MOLECULAR BIOLOGY
23 Recovery of DNA Sequences Flanking P-element Insertions:Inverse PCR and Plasmid Rescue
24 GAL4-mediated Ectopic Gene Expression in Drosophila
25 Functional Cell Ablation
GENOMICS
26 Preparation of DNA from E.coli Cells Containing Bacteriophage P1 Clones
27 Drosophila Microarrays:From Arrayer Construction to Hybridization
28 Using Drosophila Genome Databases
29 Preparation and Analysis of High-molecular-weight DNA in Drosophila
BIOCHEMISTRY
30 Culturing Large Populations of Drosophila for Protein Biochemistry
31 Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis
32 Preparation of Membrane Proteins from Drosophila Embryos
33 Preparing Cytoplasmic Extracts from Drosophila Embryos
34 Immunoblotting of Proteins from Single Drosophila Embryos
THE ORGANISM
35 Laboratory Culture of Drosophila
36 Preparation of Larval and Adult Cuticles for Light Microscopy
37 Exposing Drosophila to Neuroactive Drugs
APPENDICES
1 1989 Table of Contents from Drosophila:A Laboratory Manual
2 Anatomical Drawings of Drosophila
3 Solutions and Reagents
4 Cautions
5 Suppliers
6 Trademarks
Index
