張會圖

張會圖，男，天津科技大學生物工程學院，教授，博士生導師，北京協和醫學院博士，美國德克薩斯州農機大學（Texas A&M University）化學系博士後，中國醫藥生物技術學會理事，天津市“三年千人”第三層次人才。

基本介紹

• 中文名：張會圖
• 國籍：中國
• 學位/學歷：博士，博士後
• 專業方向：微生物藥物的篩選、分離、鑑定，以及微生物藥物（抗生素）的生物合成、代謝調控及組合生物合成研究等；工業微生物的菌種改良以及工業酶製劑的開發和產業化套用
• 職稱：教授

主講課程

本科生：《基因工程》，《微生物遺傳育種實驗技術》

參編教材

[1]《生物製藥工藝學》，人民衛生出版社。

科研方向

[1] 微生物藥物的篩選、分離、鑑定，以及微生物藥物（抗生素）的生物合成、代謝調控及組合生物合成研究等

[2] 工業微生物的菌種改良以及工業酶製劑的開發和產業化套用

科研項目

[1] 國家自然科學基金（面上項目）：非西羅黴素及阿嗪黴素中氮丙啶官能團生物合成機制的研究，2021-2024，主持人。

[3] 國家自然科學基金（面上項目）：非昔羅黴素的生物合成及組合生物合成研究，2013-2017，主持人。

[4] 國家重點研發計畫（子課題）：皮革和洗滌酶製劑智造及其綠色套用工藝，2021-2023，主持人。

[5] 轉基因生物新品種培育國家重大專項（子課題）：益生元件生物合成與調控研究，2019-2020，主持人。

[6] 國家863計畫（子課題）：工程菌小試發酵及後加工工藝研究，2012-2017，主持人。

[7] 天津市科技計畫項目：採用反轉錄PCR的方式進行安莎類抗生素的篩選，2012-2015，主持人。

[8] 企業橫向合作項目：2709鹼性蛋白酶高產菌株的構建及發酵工藝研究，2020-2021，主持人。

[9] 企業橫向合作項目：新黴素、金黴素高產菌株的選育及發酵工藝研究，2018-2020，主持人。

[10] 企業橫向合作項目：紅黴素高產菌株的選育及發酵條件最佳化，2019-2021，主持人。

[11] 企業橫向合作項目：恩拉黴素高產菌株的選育及產業化研究，2012-2015，主持人。

學術成果

先後以第一作者或通訊作者發表各類科研論文30餘篇，授權發明專利4項。部分代表性學術論文如下：

[1] Yue R, Li M, Wang Y, Guan Y, Zhang J, Yan Z, Liu F, Lu F, Zhang Huitu. Insight into enzyme-catalyzed aziridine formation mechanism in ficellomycin biosynthesis. European Journal of Medicinal Chemistry. 2020 Jul 19; 204: 112639.

[2] Yuan F, Li K, Zhou C, Zhou H, Liu H, Chai H, Lu F, Zhang Huitu. Identification of two novel highly inducible promoters from Bacillus licheniformis by screening transcriptomic data. Genomics. 2020 Mar; 112(2):1866-1871.

[3] Zhou C, Zhou H, Fang H, Ji Y, Wang H, Liu F, Zhang Huitu, Lu F. Spo0A can efficiently enhance the expression of the alkaline protease gene aprE in Bacillus licheniformis by specifically binding to its regulatory region. International Journal of Biological Macromolecules. 2020; 159:444-454.

[4] Zhang Huitu, Sang J, Zhang Y, Sun T, Liu H, Yue R, Zhang J, Wang H, Dai Y, Lu F, Liu F. Rational design of a Yarrowia lipolytica derived lipase for improved thermostability. International Journal of Biological Macromolecules. 2019; 137: 1190-1198.

[5] Zhou C, Liu H, Yuan F, Chai H, Wang H, Liu F, Li Y, Zhang Huitu, Lu F. Development and application of a CRISPR/Cas9 system for Bacillus licheniformis genome editing. International Journal of Biological Macromolecules. 2019 Feb; 122:329-337.

[6] Zhang J, He Z, Xu J, Song S, Zhu Q, Wu G, Guan Y, Wu X, Yue R, Wang Y, Yu T, Hu S, Lu F, Zhang Huitu. Semi-rational mutagenesis of an industrial Streptomyces fungicidicus strain for improved enduracidin productivity. Applied Microbiology and Biotechnology. 2020 Apr; 104(8):3459-3471.

[7] He X, Li M, Song S, Wu X, Zhang J, Wu G, Yue R, Cui H, Song S, Ma C, Lu F, Zhang Huitu. Ficellomycin: an aziridine alkaloid antibiotic with potential therapeutic capacity. Applied Microbiology and Biotechnology. 2018 May; 102(10): 4345-4354.

[8] Liu Y, Li M, Mu H, Song S, Zhang Y, Chen K, He X, Wang H, Dai Y, Lu F, Yan Z, Zhang Huitu. Identification and characterization of the ficellomycin biosynthesis gene cluster from Streptomyces ficellus. Applied Microbiology and Biotechnology. 2017 Oct; 101(20): 7589-7602.

[9] Zhou C, Zhou H, Li D, Zhang Huitu, Wang H, Lu F. Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709. Microbial Cell Factories. 2020 Feb; 19(1):45.

[10] Zhou C, Zhou H, Zhang Huitu, Lu F. Optimization of alkaline protease production by rational deletion of sporulation related genes in Bacillus licheniformis. Microbial Cell Factories. 2019 Jul; 18(1):127.

[11] Zhang Huitu, Tian Y, Wang J, Li Y, Wang H, Mao S, Liu X, Bie S, Lu F. Construction of engineered Arthrobacter simplex with improved performance for cortisone acetate biotransformation. Applied Microbiology and Biotechnology. 2013; 97(21): 9503-9514.

[12] Zhang Huitu, Sang J, Li L, Jiang L, Lu F, He S, Cui W, Zhang X, Liu F. Molecular basis for the inhibitory effects of 5-hydroxycyclopenicillone on the conformational transition of Aβ40 monomer. Journal of Biomolecular Structure and Dynamics. 2020 Jul 29: 1-12.

[13] Zhang Huitu, Liu H, Zhang Y, Sun T, Wu G, Zhou C, Wu X, Zhang J, Yue R, Wang H, Dai Y, Liu F, Lu F. Engineered variants of a lipase from Yarrowia lipolytica with improved trypsin resistance for enzyme replacement therapy. Protein Engineering Design and Selection. 2019 Dec; 32(8):375-383.

[14] Chen K, Mo Q, Liu H, Yuan F, Chai H, Lu F, Zhang Huitu. Identification and characterization of a novel cold-tolerant extracellular protease from Planococcus sp. CGMCC 8088. Extremophiles. 2018 May; 22(3):473-484.

[15] Huitu Zhang, Huiyan Mu, Qingshan Mo, Tongwei Sun, Yang Liu, Mingyu Xu, Haikuan Wang, Yujie Dai, Fuping Lu. Gene cloning, expression and characterization of a novel cold-adapted protease from Planococcus sp. Journal of Molecular Catalysis B: Enzymatic. 2016; 130: 1-8.

[16] Mori S, Simkhada D, Zhang Huitu, Erb MS, Zhang Y, Williams H, Fedoseyenko D, Russell WK, Kim D, Fleer N, Ealick SE, Watanabe CM. Polyketide ring expansion mediated by a thioesterase, chain elongation and cyclization domain, in azinomycin biosynthesis: characterization of AziB and AziG. Biochemistry. 2016; 55(4): 704-714.

[17] Lu H, Zhang Huitu, Shi P, Luo H, Wang Y, Yang P, Yao B. A family 5 β-mannanase from the thermophilic fungus Thielavia arenaria XZ7 with typical thermophilic enzyme features. Applied Microbiology and Biotechnology. 2013 Sep; 97(18): 8121-8128.

[18] Simkhada D, Zhang Huitu, Mori S, Williams H, Watanabe CM. Activation of cryptic metabolite production through gene disruption: Dimethyl furan-2,4-dicarboxylate produced by Streptomyces sahachiroi. Beilstein Journal of Organic Chemistry. 2013 Aug; 9:1768-1773.

[19] Junqi Zhao, Pengjun Shi, Yingguo Bai, Huoqing Huang, Huiying Luo, Huitu Zhang, Donghao Xu, Yaru Wang, Bin Yao. A thermophilic cellulase complex from Phialophora sp. G5 showing high capacity in cellulose hydrolysis. Applied Biochemistry and Biotechnology. 2012; 166 (4): 952-960.

[20] Huitu Zhang, linzhuan Wu, Liu Aiming, Sun Guizhi, Han Feng, Xia Huanzhang, Gao Qunjie, Yonggang Wang, Yiguang Wang. PCR screening of 3-amino- 5-hydroxybenzoic acid synthase gene leads to identification of ansamycins and AHBA related antibiotic producers in Actinomycetes. Journal of Applied Microbiology. 2009; 106(3): 755-763.

[21] Foulke-Abel J, Agbo H, Huitu Zhang, Mori S, Watanabe CM. Mode of action and biosynthesis of the azabicycle-containing natural products azinomycin and ficellomycin. Natural Product Reports. 2011; 28(4): 693-704.

發明專利

[1] 張會圖，路福平，張瑩，一種胰蛋白酶抗性改良的脂肪酶突變體及其基因和套用，CN201710441642.9

[2] 張會圖，王應東，路福平，劉東田，魏建軍. 一種恩拉黴素高產菌株的構建方法. CN2015100329738

[3] 路福平，張會圖，馮士元. 一種具備轉酯功能和耐酸抗蛋白酶特性的豬胰腺羧肽酶A1. CN201510059312

[4] 路福平，田曜，張會圖，王克鼎. 一種適用於芽孢桿菌屬同源重組的質粒及工程菌株. ZL201310262705.6